Abstract
T-lymphocytes were noted to decline for 2 acute lymphocytic leukemia (ALL) patients prior to relapse and incubation of these lymphocytes with thymosin(200ug/ml) and/or plasma increased the proportion of E-rosettes. To evaluate this induction of sheep erythrocyte(SRBC) receptors, we have studied the influence of thymosin and/or plasma on regeneration of SRBC receptors by trypsinized normal and leukemic lymphocytes.
Decreases in E-rosette forming lymphocytes were observed in two ALL patients preceeding relapse with values of 50% E-rosettes to 24% over three weeks and 58% to 9% over eight weeks. Incubation of lymphocytes from the second patient in fresh AB negative plasma for 18 hours at 37°C increased E-rosettes from 14% to 32%. After peripheral blood lymphoblasts appeared, incubation with thymosin gave values of 18% pre- and 41% post-incubation. Plasma incubation of lymphocytes from a Hodgkin's disease patient increased E-rosettes from 2% to 32%.
Eight normal subjects had E-rosettes of 60% ± 2.2 before and 6% ± 2.7 after trypsin treatment. Incubation of these lymphocytes in plasma with thymosin(61% ± 0.9) or plasma alone (68% ± 3.0) allowed complete regeneration. Thymosin alone and HBSS gave values of 46% ± 3.4 and 48% ± 4.9. Six ALL patients in remission had values of 60% ± 4.9 before and 9% ± 3.3 after trypsin treatment with regeneration >53% in all media. It is concluded that lymphocytes from ALL patients in remission after trypsinization, regenerate receptors for SRBC without thymosin or plasma factors.
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Sharpe, M., Bentley, H. & Peterson, R. 737 DECREASING T-LYMPHOCYTES BEFORE ALL RELAPSE AND in vitro STUDIES. Pediatr Res 12 (Suppl 4), 486 (1978). https://doi.org/10.1203/00006450-197804001-00742
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DOI: https://doi.org/10.1203/00006450-197804001-00742