Abstract
Human newborn adipose tissue possesses a high degree of metabolic activity. The rates of lipolysis and glycogenolysis are elevated to provide fatty acids and glucose for the increased energy demands of extrauterine life. Both of these processes are mediated in part by cyclic AMP dependent protein kinase.
The activity of cyclic AMP dependent protein kinase was determined in a group of normal infants ranging in age from 2 to 72 hrs. There was a significant increase in protein kinase activity after the first day of life, 0.23 ± 0.02 nmoles 32P incorporated/mg/min in the absence and 0.41 ± 0.02 in the presence of cyclic AMP for less than 24 hrs old and 0.29 ± 0.01 in the absence and 0.54 ± 0.02 in the presence of cyclic AMP for greater than 24 hrs. However the protein kinase activity ratio, i.e. activity —cyclic AMP/activity + cyclic AMP was highest during the first 12 hrs of life indicating that the enzyme was in a highly activated state immediately after birth. The activity ratio for 6 newborns less than 12 hrs old was 0.61 ± 0.02 compared to 0.51 ± 0.01 for 4 newborns in the 13 to 24 hr age range.
The effect of high concentrations of sodium chloride on the equilibrium between active and inactive protein kinase was also determined on the enzyme isolated from adult adipose tissue.
Supported by NIH grant no. HD04946.
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Wieser, P., Novak, M. & Buch, M. DEVELOPMENT OF CYCLIC AMP DEPENDENT PROTEIN KINASE IN HUMAN NEWBORN ADIPOSE TISSUE. Pediatr Res 11, 413 (1977). https://doi.org/10.1203/00006450-197704000-00266
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DOI: https://doi.org/10.1203/00006450-197704000-00266