Abstract
LHRH immunoreactivity in the urine has been reported with suggestions that the excreted product is intact LHRH or a 2-1O or 3-1O fragment. To better elucidate the unclear nature of urinary LHRH, we have developed radioimmunoassay systems utilizing 8 antisera. Using 3O analogs of LHRH, we tested each antiserum for binding characteristics. Three, the highly specific Niswender R-42 and the two Kelch3 antisera appeared to require the entire molecular configuration for activity; two required only 3-4 C-terminal amino acids; and three bound non-specifically to different portions of the sequence. Utilizing the sensitive Kelch13-10 antiserum, basal immunoreactive urinary LHRH in a normal man was 8 pg per ml of unconcentrated urine. After an I.V. injection of 50 μg LHRH, the concentration of urinary immunoreactive LHRH was 430 pg/ml in the first hour. An excretion rate of 101 ng/hr was found in the first hour, falling to 6.5 ng/hr by the 4th hour, with a total of 15O ng excreted in 4 hours. It is concluded that LHRH immunoreactivity is detectable in the urine in the basal state and after a bolus injection.
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Copeland, K., Cap, M., Aubert, M. et al. 66: Radioimmunoassay of urinary LHRH. Pediatr Res 10, 882 (1976). https://doi.org/10.1203/00006450-197610000-00061
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DOI: https://doi.org/10.1203/00006450-197610000-00061