Abstract
The assay of Thyroxin (T4) has been made by radio-immunoassay in the eluate of a few drops of blood deposited on a piece of blotting paper. Six disks (40 μl of blood) are eluated in 750 μl of barbital buffer for 24 hours at 4°.
The comparative assay of T4 in eluate and in fourty microliter of blood allows to find a close connection with a regressive coefficient of 0, 80. The effectivness of elution is 48 %.
The results from 6 disks 40 μl of blood) in 150 normal newborns are 600 pg ± 12.
In 5 confirmed hypothyroidians infants the average is 160±60 pg. In 2 cases of athyreosis the average is 80 pg.
This method was used for detection of hypothyroidism in one thousand of newborns by using samplings obtained from the phenylcetonuric detection center. We detected by this way a case of hypothyroidism which was confirmed by traditional tests.
This method seems to totally justifying a neonatal detection of hypothyroidism on a big scale.
Article PDF
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Rochiccioli, P., Dutau, G., Bayard, F. et al. NEONATAL DETECTION OF HYPOTHYROIDISM BY RADIOIMMUNOASSAY OF THE THYROXIN IN THE ELUATE OF DRIED BLOOD. Pediatr Res 9, 685 (1975). https://doi.org/10.1203/00006450-197508000-00109
Issue Date:
DOI: https://doi.org/10.1203/00006450-197508000-00109