Abstract
Tissue damage in jaundiced newborns is due to excessive intracellular deposits of SB. Radio-labeled SB was used to measure uptake of SB from serum by a readily accessible tissue (RBC). RBC and plasma from 0.5 ml heparinized blood were separated, and RBC resuspended in the original plasma diluted to different bilirubin/albumin ratios (B/A) with buffer and SB. After incubation, RBC's were washed and RBC cpm measured to determine RBC-B. Uptake of SB by RBC was complete in 15 minutes at all B/A (0.4-4.0). At B/A < 1, RBC-B was < 10% SB. At B/A 1.0-1.6, RBC-B increased 5-fold. RBC were saturated at B/A ≥ 2.0. Individual uptake patterns characterized each infant. Membrane-bound SB was ~ 20% of RBC-B. Newborn and adult RBC had similar affinities for SB. These results indicate that some SB enters RBC at relatively low B/A, and increases rapidly in a narrow B/A range peculiar to each infant. This direct, rapid and sensitive microassay of RBC-B may be useful in predicting the possibility of damage to newborn tissues from excessive SB.
(Supported by The Cerebral Palsy Foundation)
Article PDF
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Gotlieb, A., Applebaum, M., Thaler, M. et al. UPTAKE OF SERUM BILIRUBIN-14C (SB) BY ERYTHROCYTES CRBC) FROM NEWBORNS AND ADULTS. Pediatr Res 8, 401 (1974). https://doi.org/10.1203/00006450-197404000-00366
Issue Date:
DOI: https://doi.org/10.1203/00006450-197404000-00366