Abstract
The effect of gel-permeation on the compositional fractionation of copoly(styrene/methyl acrylate) by high performance liquid chromatography (HPLC) was studied. Mixtures of copolymer samples with different chemical compositions were fractionated by normal-phase HPLC using two columns of CN-modified silica gels with different micropores (nominal values: 10 and 30 nm) and the gradient elution of a mixture of tetrahydrofuran and cyclohexane. The samples were clearly separated into the original components according to chemical compositions by the column of 10 nm, while the separation by the column of 30 nm was very poor. The respective peaks were very low and had very long tails in the latter case. Especially, a sample of low molecular weight could not be detected. Moreover, in the chromatograms of the samples with the same chemical composition but different molecular weights by the column of 10 nm, the peak area of a sample with a molecular weight lower than 105 was half those of other samples with higher molecular weights than 105. These findings may imply that the gel-permeation effect is unfavorable to compositional fractionation, when the micropores are so large that large amounts of the copolymer samples can permeate into the micropores.
Similar content being viewed by others
Article PDF
References
H. Inagaki, Adv. Polym. Sci., 24, 190 (1977).
“Thin Layer Chromatography,” in “Fractionation of Synthetic Polymers,” L. H. Tung, Ed., Marcel Dekker, New York, N. Y., 1977.
B. G. Belenkij and E. S. Gankina, J. Chromatogr., 141, 13 (1977).
S. Teramachi, A. Hasegawa, Y. Sima, M. Akatsuka, and M. Nakajima, Macromolecules, 12, 992 (1979).
G. Riess and P. Callot, “Fractionation of Copolymer,” in “Fractionation of Synthetic Polymers,” L. H. Tung, Ed., Marcel Dekker, New York, N. Y., 1977.
G. Glöckner, H. Kroschwitz, and Ch. Meissner, Acta Polymerica, 33, 614 (1982).
M. Danielewicz and M. Kubin, J. Appl. Polym. Sci., 26, 951 (1981).
M. Danielewicz, M. Kubin, and S. Vozka, J. Appl. Polym. Sci., 27, 3629 (1982).
G. Glöckner, Pure Appl. Chem., 55, 1553 (1983).
G. Glöckner and R. Koningsveld, Makromol. Chem., Rapid Commun., 4, 529 (1983).
G. Glöckner, Jo H. M. van den Berg, N. L. J. Meijerink, T. G. Scholte, and R. Koningsveld, Macromolecules, 17, 962 (1984).
G. Glöckner, Jo H. M. van den Berg, N. L. J. Meijerink, T. G. Scholte, and R. Koningsveld, J. Chromatogr., 317, 615 (1984).
H. Sato, H. Takeuchi, S. Suzuki, and Y. Tanaka, Makromol. Chem., Rapid Commun., 5, 719 (1984).
G. Glöckner and Jo H. M. van den Berg, Chromatographia, 19, 55 (1984).
H. Sato, H. Takeuchi, and Y. Tanaka, Macromolecules, 19, 2613 (1986).
S. Mori, Y. Uno, and M. Suzuki, Anal. Chem., 58, 303 (1986).
G. Glöckner and Jo H. M. van den Berg, J. Chromatogr., 352, 511 (1986).
G. Glöckner and Jo H. M. van den Berg, J. Chromatogr., 384, 135 (1987).
G. Glöckner, J. Chromatogr., 403, 280 (1987).
S. Mori and Y. Uno, J. Appl. Polym. Sci., 34, 2689 (1987).
S. Mori and Y. Uno, Anal. Chem., 59, 90 (1987).
G. Glöckner, M. Stickler, and W. Wunderlich, Fresenius Z. Anal. Chem., 328, 76 (1987).
G. Glöckner, M. Stickler, and W. Wunderlich, Fresenius Z. Anal. Chem., 330, 46 (1988).
H. Sato, K. Mitsutani, I. Shimizu, and Y. Tanaka, J. Chromatogr., 447, 387 (1988).
S. Teramachi, A. Hasegawa, M. Akatsuka, A. Yamashita, and N. Takemoto, Macromolecules, 11, 1206 (1978).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Teramachi, S., Hasegawa, A., Shigekuni, Y. et al. Gel-Permeation Effect on Compositional Fractionation of Copoly(styrene/methyl acrylate) by High Performance Liquid Chromatography. Polym J 21, 803–808 (1989). https://doi.org/10.1295/polymj.21.803
Issue Date:
DOI: https://doi.org/10.1295/polymj.21.803