Abstract
The binding of Cu(II) by keratin proteins extracted from wool in aqueous solution has been investigated by gel filtration and equilibrium dialysis in order to compare the binding data obtained by different methods. The gel filtration binding experiments were carried out on a Sephadex G-25 gel column using 0.05 M acetate buffer as eluent in a pH range of 4.5—6.5. The elution profiles of the Cu(II)–keratin complexes were classical at equilibrium Cu(II)–concentrations from 1 to 25 ppm in the range of pH from 4.5 to 5.5. It was found that if the elution profile is classical, reliable binding data can be obtained by gel filtration with a simple technique. On the other hand, at a higher pH of 6.5, anomalous elution profiles were observed; in addition to the classical peaks, a ghost peak with respect to the Cu(II)–concentration appeared. Different mechanisms proposed for the appearance of the ghost peak by other authors were tested but failed to explain the present data. However, it was experimentally proved that the gel filtration data agreed with those of equilibrium dialysis if the Cu(II) exhibiting the ghost peak was independent of the binding by protein.
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Kadokura, S., Miyamoto, T., Ito, H. et al. Binding of Metal Ions to Wool Keratin. I. Comparison of Binding Data by Gel Filtration and Equilibrium Dialysis. Polym J 14, 121–126 (1982). https://doi.org/10.1295/polymj.14.121
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DOI: https://doi.org/10.1295/polymj.14.121
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