Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Brief Communication
  • Published:

Localization and dynamic behavior of ribosomal protein L30e

Nature Structural & Molecular Biology volume 12, pages 467–468 (2005)Cite this article

Abstract

The ribosomal protein L30e is an indispensable component of the eukaryotic 80S ribosome, where it is part of the large (60S) ribosomal subunit. Here, we determined the localization of L30e in the cryo-EM map of the 80S wheat germ (wg) ribosome at a resolution of 9.5 Å. L30e is part of the interface between large and small subunits, where it dynamically participates in the formation of the two intersubunit bridges eB9 and B4.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Figure 1: Localization of L30e in a 9.5-Å cryo-EM map of the 80S ribosome.
Figure 2: Model of L30e in its ribosomal environment.

Similar content being viewed by others

Accession codes

Accessions

Protein Data Bank

References

  1. Dabeva, M.D. & Warner, J.R. J. Biol. Chem. 268, 19669–19674 (1993).

    CAS  PubMed  Google Scholar 

  2. Vilardell, J., Yu, S.J. & Warner, J.R. Mol. Cell 5, 761–766 (2000).

    Article  CAS  PubMed  Google Scholar 

  3. Chen, Y.W., Bycroft, M. & Wong, K.B. Biochemistry 42, 2857–2865 (2003).

    Article  CAS  PubMed  Google Scholar 

  4. Chao, J.A., Prasad, G.S., White, S.A., Stout, C.D. & Williamson, J.R. J. Mol. Biol. 326, 999–1004 (2003).

    Article  CAS  PubMed  Google Scholar 

  5. Mao, H., White, S.A., Williamson, J.R. & Frankel, A.D. Nat. Struct. Biol. 6, 1139–1147 (1999).

    Article  CAS  PubMed  Google Scholar 

  6. Halic, M. et al. Nature 427, 808–814 (2004).

    Article  CAS  PubMed  Google Scholar 

  7. Rath, B.K. et al. J. Struct. Biol. 144, 95–103 (2003).

    Article  CAS  PubMed  Google Scholar 

  8. Ban, N., Nissen, P., Hansen, J., Moore, P.B. & Steitz, T.A. Science 289, 905–920 (2000).

    Article  CAS  PubMed  Google Scholar 

  9. Wimberly, B.T. et al. Nature 407, 327–339 (2000).

    Article  CAS  PubMed  Google Scholar 

  10. Klein, D.J., Schmeing, T.M., Moore, P.B. & Steitz, T.A. EMBO J. 20, 4214–4221 (2001).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Spahn, C.M. et al. Cell 107, 373–386 (2001).

    Article  CAS  PubMed  Google Scholar 

  12. Spahn, C.M. et al. EMBO J. 23, 1008–1019 (2004).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references

Acknowledgements

We acknowledge support by the VolkswagenStiftung, the European Union and Senatsverwaltung für Wissenschaft, Forschung und Kultur Berlin (UltraStructureNetwork) and the Deutsche Forschungsgemeinschaft SFB 449 (R.B.), and Howard Hughes Medical Institute (J.F.). We thank T. Mielke and R. Grassucci for assistance with F30 cryo-microscopes.

Author information

Authors and Affiliations

  1. Institute of Biochemistry, Charité, University Medical School Berlin, Monbijoustrasse 2, Berlin, 10117, Germany

    Mario Halic, Thomas Becker & Roland Beckmann

  2. Howard Hughes Medical Institute, Health Research Inc., Wadsworth Center, Empire State Plaza, Albany, 12201, New York, USA

    Joachim Frank

  3. Department of Biomedical Sciences, State University of New York at Albany, Albany, 12222, New York, USA

    Joachim Frank

  4. Institute of Medical Physics and Biophysics, Charité, University Medical School Berlin, Ziegelstrasse 9, Berlin, 10117, Germany

    Christian M T Spahn

Authors
  1. Mario Halic
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Thomas Becker
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Joachim Frank
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Christian M T Spahn
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Roland Beckmann
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Roland Beckmann.

Ethics declarations

Competing interests

The authors declare no competing financial interests.

Supplementary information

Supplementary Table 1

Binding sites and contacts of L30e and the 80S ribosome. (PDF 43 kb)

Supplementary Methods (PDF 48 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Halic, M., Becker, T., Frank, J. et al. Localization and dynamic behavior of ribosomal protein L30e. Nat Struct Mol Biol 12, 467–468 (2005). https://doi.org/10.1038/nsmb933

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/nsmb933

This article is cited by

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing