Abstract
Piwi-interacting RNAs (piRNAs) are a novel class of small RNAs that are expressed specifically and abundantly in male germ cells. Here we report that the 3′ termini of piRNAs are 2′-O-methylated; this modification may have important implications for the biogenesis and function of piRNAs.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 12 print issues and online access
$189.00 per year
only $15.75 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Bartel, D.P. Cell 116, 281–297 (2004).
Carmell, M.A., Xuan, Z., Zhang, M.Q. & Hannon, G.J. Genes Dev. 16, 2733–2742 (2002).
Aravin, A. et al. Nature 442, 203–207 (2006).
Girard, A., Sachidanandam, R., Hannon, G.J. & Carmell, M.A. Nature 442, 199–202 (2006).
Grivna, S.T., Beyret, E., Wang, Z. & Lin, H. Genes Dev. 20, 1709–1714 (2006).
Lau, N.C. et al. Science 313, 363–367 (2006).
Watanabe, T. et al. Genes Dev. 20, 1732–1743 (2006).
Deng, W. & Lin, H. Dev. Cell 2, 819–830 (2002).
Kuramochi-Miyagawa, S. et al. Development 131, 839–849 (2004).
Elbashir, S.M., Lendeckel, W. & Tuschl, T. Genes Dev. 15, 188–200 (2001).
Hutvagner, G. et al. Science 293, 834–838 (2001).
Yang, Z., Ebright, Y.W., Yu, B. & Chen, X. Nucleic Acids Res. 34, 667–675 (2006).
Vagin, V.V. et al. Science 313, 320–324 (2006).
Pelisson, A., Sarot, E., Payen-Groschene, G. & Bucheton, A. J. Virol. (2006).
Keith, G. Biochimie 77, 142–144 (1995).
Ebhardt, H.A., Thi, E.P., Wang, M.B. & Unrau, P.J. Proc. Natl. Acad. Sci. USA 102, 13398–13403 (2005).
Silberklang, M., Gillum, A.M. & RajBhandary, U.L. Methods Enzymol. 59, 58–109 (1979).
Ohara, T. et al. Nat. Struct. Mol. Biol. advance online publication 25 March 2007 (doi:10.1038/nsmb1220).
Ma, J.B., Ye, K. & Patel, D.J. Nature 429, 318–322 (2004).
Acknowledgements
We thank T. Suzuki for communicating results of unpublished experiments and T. Suzuki and members of our lab for stimulating discussions. This work was supported by grants from US National Institutes of Health (GM0720777, NS053839 and P30-HD026979) and the Philadelphia Foundation to Z.M.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Competing interests
The authors declare no competing financial interests.
Supplementary information
Supplementary Fig. 1
SYBR Gold staining of total mouse piRNAs. (PDF 228 kb)
Supplementary Fig. 2
Enzymatic sequencing of purified piR-1 and piR-1s. (PDF 570 kb)
Rights and permissions
About this article
Cite this article
Kirino, Y., Mourelatos, Z. Mouse Piwi-interacting RNAs are 2′-O-methylated at their 3′ termini. Nat Struct Mol Biol 14, 347–348 (2007). https://doi.org/10.1038/nsmb1218
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/nsmb1218
This article is cited by
-
Small RNA modifications: regulatory molecules and potential applications
Journal of Hematology & Oncology (2023)
-
Targeting Fusobacterium nucleatum through chemical modifications of host-derived transfer RNA fragments
The ISME Journal (2023)
-
Emerging roles and functional mechanisms of PIWI-interacting RNAs
Nature Reviews Molecular Cell Biology (2023)
-
PiRNA in Cardiovascular Disease: Focus on Cardiac Remodeling and Cardiac Protection
Journal of Cardiovascular Translational Research (2023)
-
Single-base resolution mapping of 2′-O-methylation sites by an exoribonuclease-enriched chemical method
Science China Life Sciences (2023)