Abstract
Histone H3 Lys4 (H3K4) is methylated by yeast Set1–COMPASS and its mammalian homolog, the MLL complex. Human JARID1d can demethylate trimethyl-H3K4 (H3K4me3). We identified Drosophila melanogaster little imaginal discs (Lid) as the JARID1d homolog. We report that Lid knockdown using RNA interference results in a specific genome-wide increase in H3K4me3 levels without affecting other patterns of H3 methylation, and results in an altered distribution of the chromo-helicase protein Chd1.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 12 print issues and online access
$189.00 per year
only $15.75 per issue
Rent or buy this article
Prices vary by article type
from$1.95
to$39.95
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Shilatifard, A. Annu. Rev. Biochem. 75, 243–269 (2006).
Miller, T. et al. Proc. Natl. Acad. Sci. USA 98, 12902–12907 (2001).
Krogan, N.J. et al. J. Biol. Chem. 277, 10753–10755 (2002).
Schneider, J. et al. Mol. Cell 19, 849–856 (2005).
Steward, M.M. et al. Nat. Struct. Mol. Biol. 13, 852–854 (2006).
Beisel, C., Imhof, A., Greene, J., Kremmer, E. & Sauer, F. Nature 419, 857–862 (2002).
Byrd, K.N. & Shearn, A. Proc. Natl. Acad. Sci. USA 100, 11535–11540 (2003).
Schneider, J. & Shilatifard, A. ACS Chem. Biol. 1, 75–81 (2006).
Shi, Y. et al. Cell 119, 941–953 (2004).
Lee, M.G. et al. Nature 437, 432–435 (2005).
Tsukuda, Y. et al. Nature 439, 811–816 (2006).
Lee, M.G., Norman, J., Shilatifard, A. & Shiekhattar, R. Cell, published online 21 February 2007.
Gildea, J.J., Lopez, R. & Shearn, A. Genetics 156, 645–663 (2000).
Flanagan, J.F. et al. Nature 438, 1181–1185 (2005).
Sims, R.J., III. et al. J. Biol. Chem. 280, 41789–41792 (2005).
Stokes, D.G., Tartof, K.D. & Perry, R.P. Proc. Natl. Acad. Sci. USA 93, 7137–7142 (1996).
Eissenberg, J.C., Shilatifard, A., Dorokhov, N. & Michener, D.E. Mol. Genet. Genomics 277, 101–114 (2007).
Van Leeuwen, F., Gafken, P.R. & Gottschling, D.E. Cell 109, 745–756 (2002).
Acknowledgements
We thank R. Perry (Fox Chase Cancer Center) for the Chd1 antiserum. J.C.E. was supported by grants from the US National Science Foundation (MCB 0131414 and MCB 0615831). R.S. was supported by a grant from the US National Institutes of Health (GM61204). A.S. was supported by grants from the US National Institutes of Health (CA089455 and GM069905).
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Competing interests
The authors declare no competing financial interests.
Supplementary information
Rights and permissions
About this article
Cite this article
Eissenberg, J., Lee, M., Schneider, J. et al. The trithorax-group gene in Drosophila little imaginal discs encodes a trimethylated histone H3 Lys4 demethylase. Nat Struct Mol Biol 14, 344–346 (2007). https://doi.org/10.1038/nsmb1217
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/nsmb1217
This article is cited by
-
Theoretical analysis of Polycomb-Trithorax systems predicts that poised chromatin is bistable and not bivalent
Nature Communications (2019)
-
Little imaginal discs, a Trithorax group member, is a constituent of nuclear matrix of Drosophila melanogaster embryos
Journal of Biosciences (2018)
-
Histone H3K4 monomethylation catalyzed by Trr and mammalian COMPASS-like proteins at enhancers is dispensable for development and viability
Nature Genetics (2017)
-
Systematic discovery of genetic modulation by Jumonji histone demethylases in Drosophila
Scientific Reports (2017)
-
The histone demethylase dKDM5/LID interacts with the SIN3 histone deacetylase complex and shares functional similarities with SIN3
Epigenetics & Chromatin (2016)