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The trithorax-group gene in Drosophila little imaginal discs encodes a trimethylated histone H3 Lys4 demethylase

Abstract

Histone H3 Lys4 (H3K4) is methylated by yeast Set1–COMPASS and its mammalian homolog, the MLL complex. Human JARID1d can demethylate trimethyl-H3K4 (H3K4me3). We identified Drosophila melanogaster little imaginal discs (Lid) as the JARID1d homolog. We report that Lid knockdown using RNA interference results in a specific genome-wide increase in H3K4me3 levels without affecting other patterns of H3 methylation, and results in an altered distribution of the chromo-helicase protein Chd1.

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Figure 1: Lid is the Drosophila homolog of JARID1d demethylase.
Figure 2: lid RNAi knockdown results in elevated H3K4me3.
Figure 3: Lid knockdown results in increased H3K4me3 on chromosomes and alters binding of Chd1.

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Acknowledgements

We thank R. Perry (Fox Chase Cancer Center) for the Chd1 antiserum. J.C.E. was supported by grants from the US National Science Foundation (MCB 0131414 and MCB 0615831). R.S. was supported by a grant from the US National Institutes of Health (GM61204). A.S. was supported by grants from the US National Institutes of Health (CA089455 and GM069905).

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Correspondence to Ali Shilatifard.

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Eissenberg, J., Lee, M., Schneider, J. et al. The trithorax-group gene in Drosophila little imaginal discs encodes a trimethylated histone H3 Lys4 demethylase. Nat Struct Mol Biol 14, 344–346 (2007). https://doi.org/10.1038/nsmb1217

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