Abstract
Recent studies have demonstrated that histone methylation can be dynamically regulated through active demethylation. However, no demethylase specific to histone H3 trimethyl-Lys4 (H3K4me3) has been identified. Here we report that the Drosophila melanogaster protein 'little imaginal discs' (Lid), a JmjC domain–containing trithorax group protein, can demethylate H3K4me3. Consistent with its genetic classification, Lid positively regulates Hox gene expression in S2 cells.
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Acknowledgements
We thank L. Fabrizio for help with mass spectrometry and S. Rogers and G. Rogers (University of North Carolina, Chapel Hill) for sharing fly reagents. This work was supported by US National Institutes of Health grants GM68804 (to Y.Z.), GM46567 (to R.S.J.) and P30 CA08748 (to P.T.). Y.Z. is an Investigator of the Howard Hughes Medical Institute. R.J.K. is funded by the Canadian Institutes of Heath Research.
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N.L. and Y.Z. designed the experiments. N.L., J.Z., R.J.K., H.E.-B. and P.T. performed the experiments. Y.Z., N.L. and R.S.J. wrote the paper.
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Supplementary information
Supplementary Fig. 1
JARID family of JmjC proteins. (PDF 243 kb)
Supplementary Fig. 2
Overexpression of Lid in S2 cells. (PDF 357 kb)
Supplementary Fig. 3
HDM activity of Lid mutant enzymes. (PDF 116 kb)
Supplementary Fig. 4
Lid distribution on polytene chromosomes. (PDF 95 kb)
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Lee, N., Zhang, J., Klose, R. et al. The trithorax-group protein Lid is a histone H3 trimethyl-Lys4 demethylase. Nat Struct Mol Biol 14, 341–343 (2007). https://doi.org/10.1038/nsmb1216
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DOI: https://doi.org/10.1038/nsmb1216
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