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Translocation: lights, camera, and action

Nature Structural & Molecular Biology volume 23, pages 367–368 (2016)Cite this article

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The fundamental mechanics of how EF-G catalyzes translocation of the mRNA and tRNA pairs on the ribosome has been intensely studied for over three decades. Two kinetic studies now reveal the sequence of events and the timing of key conformational changes in the ribosome during translocation and identify new intermediates in this complex process.

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Figure 1: Simplified model for translocation.

References

  1. Frank, J. & Agrawal, R.K. Nature 406, 318–322 (2000).

    Article  CAS  PubMed  Google Scholar 

  2. Ratje, A.H. et al. Nature 468, 713–716 (2010).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Ramrath, D.J. et al. Proc. Natl. Acad. Sci. USA 110, 20964–20969 (2013).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Tourigny, D.S., Fernández, I.S., Kelley, A.C. & Ramakrishnan, V. Science 340, 1235490 (2013).

    Article  PubMed  Google Scholar 

  5. Zhou, J., Lancaster, L., Donohue, J.P. & Noller, H.F. Science 340, 1236086 (2013).

    Article  PubMed  PubMed Central  Google Scholar 

  6. Pulk, A. & Cate, J.H. Science 340, 1235970 (2013).

    Article  PubMed  PubMed Central  Google Scholar 

  7. Valle, M. et al. Cell 114, 123–134 (2003).

    Article  CAS  PubMed  Google Scholar 

  8. Wasserman, M.R., Alejo, J.L., Altman, R.B. & Blanchard, S.C. Nat. Struct. Mol. Biol. 23, 333–341 (2016).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Belardinelli, R. et al. Nat. Struct. Mol. Biol. 23, 342–348 (2016).

    Article  CAS  PubMed  Google Scholar 

  10. Moazed, D. & Noller, H.F. Nature 342, 142–148 (1989).

    Article  CAS  PubMed  Google Scholar 

  11. Blanchard, S.C., Kim, H.D., Gonzalez, R.L. Jr., Puglisi, J.D. & Chu, S. Proc. Natl. Acad. Sci. USA 101, 12893–12898 (2004).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Munro, J.B., Altman, R.B., O'Connor, N. & Blanchard, S.C. Mol. Cell 25, 505–517 (2007).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Agirrezabala, X. et al. Mol. Cell 32, 190–197 (2008).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. Julián, P. et al. Proc. Natl. Acad. Sci. USA 105, 16924–16927 (2008).

    Article  PubMed  PubMed Central  Google Scholar 

  15. Munro, J.B., Altman, R.B., Tung, C.S., Sanbonmatsu, K.Y. & Blanchard, S.C. EMBO J. 29, 770–781 (2010).

    Article  CAS  PubMed  Google Scholar 

  16. Munro, J.B., Wasserman, M.R., Altman, R.B., Wang, L. & Blanchard, S.C. Nat. Struct. Mol. Biol. 17, 1470–1477 (2010).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  17. Brilot, A.F., Korostelev, A.A., Ermolenko, D.N. & Grigorieff, N. Proc. Natl. Acad. Sci. USA 110, 20994–20999 (2013).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  18. Schuwirth, B.S. et al. Science 310, 827–834 (2005).

    Article  CAS  PubMed  Google Scholar 

  19. Zhou, J., Lancaster, L., Donohue, J.P. & Noller, H.F. Science 345, 1188–1191 (2014).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. Chen, B. et al. Structure 23, 1097–1105 (2015).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Authors and Affiliations

  1. Xinying Shi and Simpson Joseph are at the Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California, USA.,

    Xinying Shi & Simpson Joseph

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  1. Xinying Shi
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  2. Simpson Joseph
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Correspondence to Simpson Joseph.

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The authors declare no competing financial interests.

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Shi, X., Joseph, S. Translocation: lights, camera, and action. Nat Struct Mol Biol 23, 367–368 (2016). https://doi.org/10.1038/nsmb.3219

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