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Replication origins run (ultra) deep

Nature Structural & Molecular Biology volume 19, pages 740–742 (2012)Cite this article

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The search for the holy replicator has been revitalized through ultradeep sequencing of small RNA–primed single-stranded DNA. Saturation sequencing provides an explanation for the lack of overlap in prior data sets, suggests that frequency of origin usage but not site selection is developmentally regulated, and uncovers a complex four-stranded DNA structure associated with most origins.

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Figure 1: Comparison of DNA fibers to origins demonstrates that each cell uses a different cohort of origins.
Figure 2: Ultradeep sequencing shows that origins mapped by SNS are not found throughout the genome.
Figure 3: Ultradeep sequencing captures almost all the SNS-defined origins.

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Acknowledgements

I would like to thank T. Ryba, M. Aladjem, O. Hyrien, E. Besnard and J.M. Lemaitre for assistance with assembling figures and for helpful discussions.

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  1. David M. Gilbert is in the Department of Biological Science, Florida State University, Tallahassee, Florida, USA.,

    David M Gilbert

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  1. David M Gilbert
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Correspondence to David M Gilbert.

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Gilbert, D. Replication origins run (ultra) deep. Nat Struct Mol Biol 19, 740–742 (2012). https://doi.org/10.1038/nsmb.2352

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