Abstract
Type I restriction-modification enzymes act as conventional adenine methylases on hemimethylated DNAs, but unmethylated recognition targets induce them to translocate thousands of base pairs before cleaving distant sites nonspecifically. The first crystal structure of a type I motor subunit responsible for translocation and cleavage suggests how the pentameric translocating complex is assembled and provides a structural framework for translocation of duplex DNA by RecA-like ATPase motors.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 12 print issues and online access
$189.00 per year
only $15.75 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
Accession codes
References
Murray, N.E. Microbiol. Mol. Biol. Rev. 64, 412–434 (2000).
Szczelkun, M.D., Dillingham, M.S., Janscak, P., Firman, K. & Halford, S.E. EMBO J. 15, 6335–6347 (1996).
Seidel, R. et al. Nat. Struct. Mol. Biol. 11, 838–843 (2004).
Stanley, L.K. et al. EMBO J. 25, 2230–2239 (2006).
Seidel, R., Bloom, J.G., Dekker, C. & Szczelkun, M.D. EMBO J. 27, 1388–1398 (2008).
Niv, M.Y. et al. Nucleic Acids Res. 35, 2227–2237 (2007).
Sengoku, T., Nureki, O., Nakamura, A., Kobayashi, S. & Yokoyama, S. Cell 125, 287–300 (2006).
Powell, L.M., Dryden, D.T.F. & Murray, N.E. J. Mol. Biol. 283, 963–976 (1998).
Mernagh, D.R., Janscak, P., Firman, K. & Kneale, G.G. Biol. Chem. 379, 497–503 (1998).
Dürr, H., Körner, C., Müller, M., Hickmann, V. & Hopfner, K.P. Cell 121, 363–373 (2005).
Dürr, H., Flaus, A., Owen-Hughes, T. & Hopfner, K.P. Nucleic Acids Res. 34, 4160–4167 (2006).
Lee, J.Y. & Yang, W. Cell 127, 1349–1360 (2006).
Lewis, R., Dürr, H., Hopfner, K.P. & Michaelis, J. Nucleic Acids Res. 36, 1881–1890 (2008).
Davies, G.P. et al. J. Mol. Biol. 290, 565–579 (1999).
Makovets, S., Powell, L.M., Titheradge, A.J., Blakely, G.W. & Murray, N.E. Mol. Microbiol. 51, 135–147 (2004).
van Noort, J., van der Heijden, T., Dutta, C.F., Firman, K. & Dekker, C. Nucleic Acids Res. 32, 6540–6547 (2004).
Acknowledgements
We gratefully acknowledge support from the Ministry of Education, Youth and Sports of the Czech Republic (MSM6007665808, LC06010), Academy of Sciences of the Czech Republic (AVOZ60870520), Grant Agency of the Czech Republic (203/08/0114 to R.E), the European Molecular Biology Organization (to M.L.), the Swiss National Science Foundation (to P.J.) and joint Czech and US National Science Foundation International Research Cooperation (INT03-09049 to J.C.). We thank the European Molecular Biology Laboratory for access to the X12 beamline at the DORIS storage ring, DESY, Hamburg. J.C. and R.E. thank F. Hughson, T. Lohman, B. Matthews, L. Raleigh and S. Weller for insightful discussion during preparation of the manuscript.
Author information
Authors and Affiliations
Corresponding authors
Supplementary information
Supplementary Text and Figures
Supplementary Figures 1 and 2, Supplementary Table 1 and Supplementary Methods (PDF 2292 kb)
Rights and permissions
About this article
Cite this article
Lapkouski, M., Panjikar, S., Janscak, P. et al. Structure of the motor subunit of type I restriction-modification complex EcoR124I. Nat Struct Mol Biol 16, 94–95 (2009). https://doi.org/10.1038/nsmb.1523
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/nsmb.1523
This article is cited by
-
Molecular insights into DNA recognition and methylation by non-canonical type I restriction-modification systems
Nature Communications (2022)
-
A residue of motif III positions the helicase domains of motor subunit HsdR in restriction-modification enzyme EcoR124I
Journal of Molecular Modeling (2018)
-
Interdomain communication in the endonuclease/motor subunit of type I restriction-modification enzyme EcoR124I
Journal of Molecular Modeling (2014)
-
In silico characterization of the motor subunit of the e.coli. restriction-modification system EcoR1241
Journal of Cheminformatics (2013)