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Training ribozymes to switch

Abstract

Ribozymes that are sensitive to cyclic nucleotides have been selected in vitro. Remarkably, the cGMP-dependent ribozymes are specifically activated by a factor of 5,000 — the largest allosteric ribozyme activation by a small molecule seen to date.

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Figure 1: Aptamer selection versus aptazyme selection.
Figure 2: Progression of selection.
Figure 3: Aptazyme chips.

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Correspondence to Andrew D. Ellington.

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Marshall, K., Ellington, A. Training ribozymes to switch. Nat Struct Mol Biol 6, 992–994 (1999). https://doi.org/10.1038/14872

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