Abstract
The protein hRPABC14.4 is an essential subunit of human RNA polymerases I, II, and III and is required for the transcription of all human nuclear genes. The structure of hRPABC14.4 was determined by nuclear magnetic resonance spectroscopy. The protein fold comprises a highly conserved central domain forming two antiparallel α-helices flanked by the less conserved N- and C-terminal regions forming a five-stranded β-sandwich. Amino acids from the two helices participate in the generation of a hydrophobic surface area which is conserved in all eukaryotic and archaeal homologous subunits, and likely constitutes a critical macromolecular interaction interface. The hRPABC14.4 structure accounts for mutagenesis results in Saccharomyces cerevisiae and provides a structural working model for elucidating the role of this subunit in the molecular architecture and function of the human nuclear RNA polymerases.
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Acknowledgements
Supported by grants from the American Heart Association and National Institutes of Health (J.A.A.L.), and grants from the National Institutes of Health and National Science Foundation (G.W.). J.A.A.L. is an Established Investigator of the American Heart Association.
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del Río-Portilla, F., Gaskell, A., Gilbert, D. et al. Solution structure of the hRPABC14.4 subunit of human RNA polymerases. Nat Struct Mol Biol 6, 1039–1042 (1999). https://doi.org/10.1038/14923
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DOI: https://doi.org/10.1038/14923