Abstract
Crystallization of membrane proteins, a prerequisite for their X-ray crystallographic analysis, remains difficult. Here, we demonstrate that the crystallization of the cytochrome c oxidase from Paracoccus denitrificans can be mediated by co-crystallization with an antibody Fv fragment. The crystals obtained contain all four subunits of this membrane protein complex and the Fv fragment. The approach of co-crystallizing membrane proteins with antibody fragments should be useful in obtaining well-ordered crystals of membrane proteins in general.
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Ostermeier, C., Iwata, S., Ludwig, B. et al. Fv fragment-mediated crystallization of the membrane protein bacterial cytochrome c oxidase. Nat Struct Mol Biol 2, 842–846 (1995). https://doi.org/10.1038/nsb1095-842
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DOI: https://doi.org/10.1038/nsb1095-842
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