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Fv fragment-mediated crystallization of the membrane protein bacterial cytochrome c oxidase

Nature Structural Biology volume 2, pages 842–846 (1995)Cite this article

Abstract

Crystallization of membrane proteins, a prerequisite for their X-ray crystallographic analysis, remains difficult. Here, we demonstrate that the crystallization of the cytochrome c oxidase from Paracoccus denitrificans can be mediated by co-crystallization with an antibody Fv fragment. The crystals obtained contain all four subunits of this membrane protein complex and the Fv fragment. The approach of co-crystallizing membrane proteins with antibody fragments should be useful in obtaining well-ordered crystals of membrane proteins in general.

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Authors and Affiliations

  1. Max-Planck-lnstitut für Biophysik, Heinrich-Hoffmann-Str. 7, D-60528, Frankfurt/Main, Germany

    Christian Ostermeier, So Iwata & Hartmut Michel

  2. Johann Wolfgang Goethe-Universität, Biozentrum N200, Institut für Biochemie, Molekulare Genetik, Marie-Curie-Str. 9, D 60439, Frankfurt/M., Germany

    Bernd Ludwig

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  1. Christian Ostermeier
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Ostermeier, C., Iwata, S., Ludwig, B. et al. Fv fragment-mediated crystallization of the membrane protein bacterial cytochrome c oxidase. Nat Struct Mol Biol 2, 842–846 (1995). https://doi.org/10.1038/nsb1095-842

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