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Crystal structure of RhoA–GDP and its functional implications

Nature Structural Biology volume 4, pages 699–703 (1997)Cite this article

RhoA, a ubiquitous intracellular GTPase, mediates cytoskeletal responses to extracellular signals. A 2.1 Å resolution crystal structure of the human RhoA–GDP complex shows unique stereochemistry in the switch I region, which results in a novel mode of Mg2+ binding.

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References

  1. Hall, A. Ann. Rev. Cell Biol. 10, 31–54 (1994).

    Article  CAS  PubMed  Google Scholar 

  2. Burridge, K. & Chrzanowska-Wodnicka, M. Ann. Rev. Cell Dev. Biol. 12, 463–518 (1996).

    Article  CAS  Google Scholar 

  3. Narumiya, S. J. Biochem. 120, 215–228 (1996).

    Article  CAS  PubMed  Google Scholar 

  4. Hill, C.S., Wynne, J. & Treisman, R. Cell 81, 1159–70 (1995).

    Article  CAS  PubMed  Google Scholar 

  5. Norman, J.C., Price, L.S., Ridley, A.J. & Koffer, A. Mol. Biol.Cell, 7, 1429–1442 (1996).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Gong, M.C. et al. Proc. Natl. Acad. Sci. USA 93, 1340–5 (1996).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Zhang, J. et al. J. Biol. Chem. 270, 6589–94 (1995).

    Article  CAS  PubMed  Google Scholar 

  8. Harhammer, R., Gohla, A. & Schultz, G. FEBS Lett. 399, 211–214 (1996).

    Article  CAS  PubMed  Google Scholar 

  9. Nobes, C.D., Hawkins, P., Stephens, L. & Hall, A. J. Cell Sci. 108, 225–33 (1995).

    CAS  PubMed  Google Scholar 

  10. Kwak, J.Y., Lopez, I., Uhlinger, D.J., Ryu, S.H. & Lambeth, J.D. J. Biol. Chem. 270, 27093–8 (1995).

    Article  CAS  PubMed  Google Scholar 

  11. Siddiqi, A.R. et al. J. Biol. Chem. 270, 8466–73 (1995).

    Article  CAS  PubMed  Google Scholar 

  12. Somlyo, A.P. & Somlyo, A.V. Nature 372, 231–6 (1994).

    Article  CAS  PubMed  Google Scholar 

  13. Lim, L., Manser, E., Leung, T. & Hall, C. Eur. J. Biochem. 242, 171–185 (1996).

    Article  CAS  PubMed  Google Scholar 

  14. Kimura, K. et al. Science 273, 245–248 (1996).

    Article  CAS  PubMed  Google Scholar 

  15. Hall, A. Mol. Biol.Cell 3, 475–9 (1992).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  16. Bokoch, G.M., Bohl, B.P. & Chuang, T.H. J. Biol. Chem. 269, 31674–9 (1994).

    CAS  PubMed  Google Scholar 

  17. Gong, M.C., Fujihara, H., Walker, L.A., Somlyo, A.V. & Somlyo, A.P. Mol. Biol. Cell 8, 279–286 (1997).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  18. Gong, M.C., Fujihara, H., Somlyo, A.V. & Somlyo, A.P. J. Biol. Chem. 272, 10704–10709 (1997).

    Article  CAS  PubMed  Google Scholar 

  19. Hirshberg, M., Stockley, R., Dodson, G. & Webb, R. Nature Struct. Biol. 4, 147–151 (1997).

    Article  CAS  PubMed  Google Scholar 

  20. Lambright, D.G., Noel, J.P., Hamm, H.E. & Sigler, P.B. Nature 369, 621–628 (1994).

    Article  CAS  PubMed  Google Scholar 

  21. John, J. et al. J. Biol. Chem. 268, 923–9 (1993).

    CAS  PubMed  Google Scholar 

  22. Li, R. & Zheng, Y. J. Biol. Chem. 272, 4671–4679 (1997).

    Article  CAS  PubMed  Google Scholar 

  23. Selzer, J. et al. J. Biol. Chem. 271, 25173–7 (1996).

    Article  CAS  PubMed  Google Scholar 

  24. Kawashima, T., Berthet-Colominas, C., Wulff, M., Cusack, S. & Leberman, R. Nature 379, 511–8 (1996).

    Article  CAS  PubMed  Google Scholar 

  25. Rubin, E.J., Gill, D.M., Boquet, P. & Popoff, M.R. Mol. Cell. Biol. 8, 418–26 (1988).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  26. Sugai, M., Chen, C.H. & Wu, H.C. Proc. Natl. Acad. Sci. USA 89, 8903–7 (1992).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  27. Kraulis, P.J., Domaille, P.J., Campbell-Burk, S.L., Van Aken, T. & Laue, E.D. Biochemistry 33, 3515–31 (1994).

    Article  CAS  PubMed  Google Scholar 

  28. Paterson, H.F. et al. J. Cell Biol. 111, 1001–7 (1990).

    Article  CAS  PubMed  Google Scholar 

  29. Jones, T.A., Zou, J.Y., Cowan, S.W. & Acta Crystallogr. A47, 110–9 (1991).

    Article  CAS  Google Scholar 

  30. Laskowski, R.A., McArthur, M.W., Moss, D.S. & Thornton, J.M. J. Appl. Crystallogr. 26, 282–291 (1993).

    Article  Google Scholar 

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Authors and Affiliations

  1. Department of Molecular Physiology and Biological Physics, Center for Structural Biology, University of Virginia Health Sciences Center, P.O. Box 10011, Charlottesville, Virginia, 22906-0011, USA

    Yunyi Wei, Yan Zhang, Urszula Derewenda, Xiaopu Liu, Wladek Minor, Robert K. Nakamoto, Avril V. Somlyo, Andrew P. Somlyo & Zygmunt S. Derewenda

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  1. Yunyi Wei
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Wei, Y., Zhang, Y., Derewenda, U. et al. Crystal structure of RhoA–GDP and its functional implications. Nat Struct Mol Biol 4, 699–703 (1997). https://doi.org/10.1038/nsb0997-699

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