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Thinking outside the box: new insights into the mechanism of GroEL-mediated protein folding

Nature Structural Biology volume 6, pages 597–600 (1999)Cite this article

Abstract

GroEL is thought to assist protein folding in part by providing a passive 'box' in which folding can occur without danger of aggregation. Two new papers now suggest that GroEL can also play a more active role by promoting rearrangement and even unfolding of kinetic folding intermediates.

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Figure 1: Schematic of the GroEL reaction cycle.
Figure 2: Hypothetical 'kinetic-editing' model for selection of GroEL substrates.
Figure 3: A model for GroES and ATP-dependent unfolding.

References

  1. Sigler, P.B. et al. Annu. Rev. Biochem. 67, 581–608 (1998).

    Article  CAS  Google Scholar 

  2. Rye, H.S. et al. Cell 97, 325–38 (1999).

    Article  CAS  Google Scholar 

  3. Peralta, D., Hartman, D.J., Hoogenraad, N.J. & Hoj, P.B. FEBS Lett. 339, 45–49 (1994).

    Article  CAS  Google Scholar 

  4. Sparrer, H., Rutkat, K. & Buchner, J. Proc. Natl. Acad. Sci. USA 94, 1096–100 (1997).

    Article  CAS  Google Scholar 

  5. Todd, M.J., Viitanen, P.V. & Lorimer, G.H. Science 265, 659–666 (1994).

    Article  CAS  Google Scholar 

  6. Coyle, J.E. et al. Nature Struct. Biol. 6, 683–690 (1999).

    Article  CAS  Google Scholar 

  7. Shtilerman, M., Lorimer, G.H. & Englander, S.W. Science 284, 822–825 (1999).

    Article  CAS  Google Scholar 

  8. Radford, S.E. & Dobson, C.M. Phil. Trans. R. Soc. Lond. B 348, 17–25 (1995).

    Article  CAS  Google Scholar 

  9. Ewalt, K.L., Hendrick, J.P., Houry, W.A. & Hartl, F.U. Cell 90, 491–500 (1997).

    Article  CAS  Google Scholar 

  10. Lorimer, G.H. FASEB J. 10, 5–9 (1996).

    Article  CAS  Google Scholar 

  11. Ellis, R.J. & Hartl, F.U. FASEB J. 10, 20–26 (1996).

    Article  CAS  Google Scholar 

  12. Dill, K.A. & Chan, H.S. Nature Struct. Biol. 4, 10–9 (1997).

    Article  CAS  Google Scholar 

  13. Sosnick, T.R., Mayne, L., Hiller, R. & Englander, S.W. Nature Struct. Biol. 1, 149–156 (1994).

    Article  CAS  Google Scholar 

  14. Weissman, J.S. & Kim, P.S. Science 253, 1386–1393 (1991).

    Article  CAS  Google Scholar 

  15. Todd, M.J., Lorimer, G.H. & Thirumalai, D. Proc. Natl. Acad. Sci. USA 93, 4030–5 (1996).

    Article  CAS  Google Scholar 

  16. Corrales, F.J. & Fersht, A.R. Proc. Natl. Acad. Sci. USA 93, 4509–12 (1996).

    Article  CAS  Google Scholar 

  17. Goloubinoff, P., Gatenby, A.A. & Lorimer, G.H. Nature 337, 44–47 (1989).

    Article  CAS  Google Scholar 

  18. Goloubinoff, P., Christeller, J.T., Gatenby, A.A. & Lorimer, G.H. Nature 342, 884–889 (1989).

    Article  CAS  Google Scholar 

  19. Weissman, J.S., Kashi, Y., Fenton, W.A. & Horwich, A.L. Cell 78, 693–702 (1994).

    Article  CAS  Google Scholar 

  20. Zahn, R., Perrett, S., Stenberg, G. & Fersht, A.R. Science 271, 642–645 (1996).

    Article  CAS  Google Scholar 

  21. Walter, S., Lorimer, G.H. & Schmid, F.X. Proc. Natl. Acad. Sci. USA 93, 9425–30 (1996).

    Article  CAS  Google Scholar 

  22. Weissman, J.S., Rye, H.S., Fenton, W.A., Beechem, J.M. & Horwich, A.L. Cell 84, 481–490 (1996).

    Article  CAS  Google Scholar 

  23. Rye, H.S. et al. Nature 388, 792–8 (1997).

    Article  CAS  Google Scholar 

  24. Braig, K. et al. Nature 371, 578–586 (1994).

    Article  CAS  Google Scholar 

  25. Xu, Z., Horwich, A.L. & Sigler, P.B. Nature 388, 741–50 (1997).

    Article  CAS  Google Scholar 

Download references

Acknowledgements

We thank G. Lorimer and P. Sigler for Fig. 3, and members of the Weissman lab for helpful comments.

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Authors and Affiliations

  1. the Department of Cellular & Molecular Pharmacology, University of California- San Francisco, 513 Parnassus Ave, San Francisco, 94143-0450, California, USA

    Jue D. Wang & Jonathan S. Weissman

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  1. Jue D. Wang
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  2. Jonathan S. Weissman
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Correspondence to Jonathan S. Weissman.

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Wang, J., Weissman, J. Thinking outside the box: new insights into the mechanism of GroEL-mediated protein folding. Nat Struct Mol Biol 6, 597–600 (1999). https://doi.org/10.1038/10636

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