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Dipolar recoupling in MAS spectra of biological solids

Nature Structural Biology volume 5, pages 508–512 (1998)Cite this article

Abstract

In combination with magic angle spinning, dipolar recoupling yields solid state NMR spectral assignments and provides constraints on internuclear distances and torsion angles. The method offers a fresh approach to structural studies of a variety of systems that cannot be examined with conventional tools available to structural biology.

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Figure 1: Schematic illustration of the basic of the basic physics of dipolar recoupling during MAS.
Figure 2
Figure 3
Figure 4: Extraction of the NCCN (ψ) torsion angle in glycylglycine•HCI (diluted 1:40 in natural abundance).
Figure 5: DNP-CP 13C solid state MAS (3.0 kHz spin rate) spectra of fully 13C-15N-labeled L-Arg (30 mg ml–1 Arg and 40 mM 4-amino-TEMPO in 60:40 water-glycerol) at 55 K.

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  1. Francis Bitter Magnet Laboratory and Department of Chemistry, Massachusetts Institute of Technology, Cambridge,, 02139-4507, MA, USA

    Robert Griffin

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Correspondence to Robert Griffin.

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Griffin, R. Dipolar recoupling in MAS spectra of biological solids. Nat Struct Mol Biol 5 (Suppl 7), 508–512 (1998). https://doi.org/10.1038/749

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