Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • News & Views
  • Published:

Restriction endonucleases: one of these things is not like the others

Nature Structural Biology volume 7, pages 89–91 (2000)Cite this article

Abstract

The crystal structure of the restriction endonuclease BglII in complex with its DNA target site has been determined. The DNA binding mode and chemistry of catalysis are observed to differ from BamHI which cleaves a similar target site. These observations indicate that more divergence has occurred within this family of proteins than originally thought.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Figure 1
Figure 2: Ribbon diagrams of protein–DNA complexes of BamHI, BglII, and I-CreI.

References

  1. Lukacs, C., Kucera, R., Schildkraut, I. & Aggarwal, A.K. Nature Struct. Biol. 7, 134–140 (2000).

    Article  CAS  Google Scholar 

  2. Viadiu, H. & Aggarwal, A.K. Nature Struct. Biol. 5, 910–916 (1998).

    Article  CAS  Google Scholar 

  3. Winkler, F. et al. EMBO J. 12, 1781–1795 (1993).

    Article  CAS  Google Scholar 

  4. Kostrewa, D. & Winkler, F.K. Biochemistry 34, 683–696 (1995).

    Article  CAS  Google Scholar 

  5. Alves, J. et al. Biochemistry 28, 2678– 2684 (1989).

    Article  CAS  Google Scholar 

  6. Wenz, C. et al. Biochem. Biophys. Acta 1219, 73– 80 (1994).

    CAS  PubMed  Google Scholar 

  7. Ivanenko, T. Heitman, J. and Kiss, A. Biol. Chem. 379, 459–465 (1998).

    Article  CAS  Google Scholar 

  8. Dorner, L F., Bitinaite, J., Whitaker, R. D. & Schildraut, I. J. Mol. Biol. 285, 1515–1523 (1999).

    Article  CAS  Google Scholar 

  9. Pingoud, A. & Jeltsch, A. Eur. J. Biochem. 246 , 1–22 (1997).

    Article  CAS  Google Scholar 

  10. Heath, P.J. Stephens, K. M., Monnat, R. J. Jr. & Stoddard, B.L. Nature Struct. Biol. 4, 468 –476 (1997).

    Article  CAS  Google Scholar 

  11. Jurica, M.S., Monnat, R. J. Jr. & Stoddard, B.L. Mol. Cell 2, 469–476 (1998).

    Article  CAS  Google Scholar 

  12. Belfort, M. & Roberts R.J. Nucleic Acids Res. 25, 3379–3388 (1997).

    Article  CAS  Google Scholar 

  13. Argast, G.M., Stephens, K. M., Emond, M. J. & Monnat R.J. Jr., J. Mol. Biol. 280, 345– 353 (1998).

    Article  CAS  Google Scholar 

  14. Deibert, M., Grazulis, S., Janulaitis, A., Siksnys, V. & Huber, R. EMBO J. 18, 5805–5816 (1999).

    Article  CAS  Google Scholar 

Download references

Acknowledgements

E.A.G. is supported by an NIH training grant fellowship; B.L.S. is funded for studies in this field by the NIH. The authors thank C. Lukacs and A. Aggarwal for providing coordinates prior to publication and release and for comments on this summary.

Author information

Authors and Affiliations

  1. the Program in Biomolecular Structure and Design, University of Washington and the Fred Hutchinson Cancer Research Center, Seattle, 98109, 1100 Fairview Ave. N., Washington, USA

    Eric A. Galburt & Barry L. Stoddard

Authors
  1. Eric A. Galburt
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Barry L. Stoddard
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Barry L. Stoddard.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Galburt, E., Stoddard, B. Restriction endonucleases: one of these things is not like the others . Nat Struct Mol Biol 7, 89–91 (2000). https://doi.org/10.1038/72450

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1038/72450

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing