The structure of the gyrA intein provides a view of its active site prior to the initial step of the protein splicing pathway. An intact leaving group at the N-terminal splice junction displays a highly strained cis geometry that is potentially critical for driving the isomerization of the scissile peptide bond, inducing subsequent cleavage.
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Stoddard, B., Pietrokovski, S. Breaking up is hard to do. Nat Struct Mol Biol 5, 3–5 (1998). https://doi.org/10.1038/nsb0198-3
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DOI: https://doi.org/10.1038/nsb0198-3
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