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In search of the holy replicator

Nature Reviews Molecular Cell Biology volume 5, pages 848–855 (2004)Cite this article

Abstract

After 40 years of searching for the eukaryotic replicator sequence, it is time to abandon the concept of 'the' replicator as a single genetic entity. Here I propose a 'relaxed replicon model' in which a positive initiator–replicator interaction is facilitated by a combination of several complex features of chromatin. An important question for the future is whether the positions of replication origins are simply a passive result of local chromatin structure or are actively localized to coordinate replication with other chromosomal activities.

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Figure 1: The replicon model.
Figure 2: Relaxed replicon model.

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Acknowledgements

I would like to apologize to those whose work was not cited due to space limitations. I would also like to thank R. Cross, C. Cvetic, J. Huberman, P. Kane, T. Melendy, C. Schildkraut, J. Walter and R. West for critical reading of the manuscript. D.M.G. is supported by the National Institutes of Health, the National Science Foundation and American Cancer Society.

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  1. the Department of Biochemistry and Molecular Biology, State University of New York Upstate Medical University, 750 East Adams Street, Syracuse, 13210, NewYork, USA

    David M. Gilbert

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DATABASES

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DHFR

dnaA

HBB

oriC

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Myb

Rpd3

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Gilbert, D. In search of the holy replicator. Nat Rev Mol Cell Biol 5, 848–855 (2004). https://doi.org/10.1038/nrm1495

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