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Functional immunoimaging: the revolution continues

Nature Reviews Immunology volume 12, pages 858–864 (2012)Cite this article

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Abstract

Ten years ago, in 2002, the introduction of dynamic in vivo imaging to immunologists set a new standard for studying immune responses. In particular, two-photon imaging has provided tremendous insights into immune cell dynamics in various contexts, including infection, cancer, transplantation and autoimmunity. Whereas initial studies were restricted to the migration of and interactions between immune cells, recent advances are bringing intravital imaging to a new level in which cell dynamics and function can be investigated simultaneously. These exciting developments further broaden the applications of immunoimaging and provide unprecedented opportunities to probe and decode immune cell communication in situ.

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Figure 1: Illuminating the many facets of the immune system.
Figure 2: The expanding toolbox of immunoimaging.
Figure 3: Combining imaging and phenotyping.

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Acknowledgements

We apologize to our colleagues for not being able to mention all of the important contributions that have been made in this field owing to space limitations. We wish to thank members of the Bousso laboratory for generating data contributing to this article. This work is supported by the Institut Pasteur, INSERM, the Fondation pour la Recherche Médicale and the European Research Council starting grant 'LymphocyteContact'.

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Authors and Affiliations

  1. Philippe Bousso and Hélène D. Moreau are at the Institut Pasteur, Dynamics of Immune Responses Unit, F-75015 Paris, France.,

    Philippe Bousso & Hélène D. Moreau

  2. Philippe Bousso and Hélène D. Moreau are also at INSERM U668, Rue du Docteur Roux, F-75015 Paris, France.,

    Philippe Bousso & Hélène D. Moreau

  3. Hélène D. Moreau is also at the Université Paris Diderot, Sorbonne Paris Cité, Cellule Pasteur, Rue du Docteur Roux, F-75015 Paris, France.,

    Hélène D. Moreau

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Correspondence to Philippe Bousso.

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Bousso, P., Moreau, H. Functional immunoimaging: the revolution continues. Nat Rev Immunol 12, 858–864 (2012). https://doi.org/10.1038/nri3342

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