Abstract
The Barlow-Hall method for in vitro evolution is a simple alternative to methods such as DNA shuffling and is particularly applicable to predicting the evolution of target genes in nature. This detailed protocol includes the procedure itself and, as Appendices, describes particularly effective protocols for electroporation and for preparation of electrocompetent cells.
Similar content being viewed by others
Article PDF
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hall, B., Barlow, M. Barlow-Hall in vitro Evolution Protocol. Nat Prec (2008). https://doi.org/10.1038/npre.2008.1772.1
Received:
Accepted:
Published:
DOI: https://doi.org/10.1038/npre.2008.1772.1