Letter

Extrafloral nectar secretion from wounds of Solanum dulcamara

  • Nature Plants 2, Article number: 16056 (2016)
  • doi:10.1038/nplants.2016.56
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Abstract

Plants usually close wounds rapidly to prevent infections and the loss of valuable resources such as assimilates1. However, herbivore-inflicted wounds on the bittersweet nightshade Solanum dulcamara appear not to close completely and produce sugary wound secretions visible as droplets. Many plants across the plant kingdom secrete sugary nectar from extrafloral nectaries2 to attract natural enemies of herbivores for indirect defence3,4. As ants forage on wound edges of S. dulcamara in the field, we hypothesized that wound secretions are a form of extrafloral nectar (EFN). We show that, unlike EFN from known nectaries, wound secretions are neither associated with any specific structure nor restricted to certain locations. However, similar to EFN, they are jasmonate-inducible and the plant controls their chemical composition. Wound secretions are attractive for ants, and application of wound secretion mimics increases ant attraction and reduces herbivory on S. dulcamara plants in a natural population. In greenhouse experiments, we reveal that ants can defend S. dulcamara from two of its native herbivores, slugs and flea beetle larvae. Since nectar is defined by its ecological function as a sugary secretion involved in interactions with animals5, such ‘plant bleeding’ could be a primitive mode of nectar secretion exemplifying an evolutionary origin of structured extrafloral nectaries.

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Acknowledgements

We thank our students J. Schößler and M. Wank for help during experiments, A. Erban for assistance on the analysis of the GC–MS chromatograms, R. Radek for supporting SEM measurements and T. Sonsalla for help with figure art work. We thank the Freie Universität Berlin for funding as well as the German Research Foundation (DFG) for financial support to establish a German–Dutch cooperation (DFG: STE_2014_1_1). O.W.C. was funded by a grant of the bilateral agreement for higher education (Erasmus) between the Freie Universität Berlin and Radboud University, and a stipend of the CRC973 funded by the DFG. A.S. acknowledges funding by the CRC973 (project B2) and N.M.v.D. gratefully acknowledges the support of the German Centre for Integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig funded by the German Research Foundation (FZT 118).

Author information

Affiliations

  1. Molecular Ecology, Dahlem Centre of Plant Sciences, Institute of Biology/Freie Universität Berlin, Haderslebener Strasse 9, 12163 Berlin, Germany

    • Tobias Lortzing
    • , Onno W. Calf
    • , Marlene Böhlke
    • , Daniel Geuß
    • , Susanne Kosanke
    •  & Anke Steppuhn
  2. Molecular Interaction Ecology, Institute of Water and Wetland Research, Radboud University, PO Box 9010, 6500 GL Nijmegen, The Netherlands

    • Onno W. Calf
    •  & Nicole M. van Dam
  3. Applied Metabolome Analysis, Max-Planck-Institute of Molecular Plant Physiology, Am Mühlenberg 1, 14476 Potsdam, Germany

    • Jens Schwachtje
    •  & Joachim Kopka
  4. German Centre for Integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig, Deutscher Platz 5e, 04103 Leipzig, Germany

    • Nicole M. van Dam
  5. Institute of Ecology, Friedrich Schiller University Jena, Dornburger-Strasse. 159, 07743 Jena, Germany

    • Nicole M. van Dam

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Contributions

T.L. and A.S. designed this study. The experimental work was planned by T.L., A.S., O.W.C., N.M.v.D., and for metabolite analysis also by J.K. Experiments and analyses were conducted by T.L., O.W.C., M.B., D.G., S.K. and additionally by J.S. and J.K. for the metabolite analysis. T.L. and A.S. wrote the first draft of the manuscript that was edited and revised by all authors.

Competing interests

The authors declare no competing financial interests.

Corresponding author

Correspondence to Anke Steppuhn.

Supplementary information

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    Supplementary Information

    Supplementary Materials and Methods, Supplementary Tables 1–4 and Supplementary Figs 1–5.

Excel files

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    Supplementary Dataset