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Putative RopGAPs impact division plane selection and interact with kinesin-12 POK1

Nature Plants volume 2, Article number: 16120 (2016) Cite this article

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Abstract

Cell shape is defined by the surrounding cell walls in plants. Thus, spatial control over cell division planes and cell expansion polarity are essential to maintain cell morphology. In eukaryotes, cell polarity and expansion are controlled by Rho GTPase signalling, regulating cytoskeletal reorganization and vesicle trafficking1. However, until now, Rho signalling was not implicated in mitotic events in plants. Here, we report a pair of putative Rho GTPase activating proteins (RhoGAPs) that interact with the mitosis-specific kinesin-12 POK1, a core component of the cortical division zone/site (CDZ/CDS) that is required for division plane maintenance in Arabidopsis2–4. The designated pleckstrin homology GAPs (PHGAPs) are cytoplasmic and plasma membrane associated in interphase, but during mitosis they additionally localize to the CDZ/CDS in a POK-dependent manner. In contrast to pok1 pok2 mutants, phgap1 phgap2 double mutants show moderate cell wall positioning defects as a consequence of inaccurate positioning of the cortical division zone marker POK1. We conclude that loss of PHGAP function interferes with division plane selection in proliferative cell divisions.

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Figure 1: Organization of PHGAP protein domains and protein interaction assays.
Figure 2: PHGAP accumulation at the cortical division zone is cell-cycle- and POK-dependent.
Figure 3: Phenotype analysis of phgap1 phgap2 mutants compared with the wild type.
Figure 4: Mitotic microtubule array orientation and POK1 ring position.

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Acknowledgements

We thank M. Singh for helpful discussion and C. Grefen for discussion and reagents. Funding was provided by the Deutsche Forschungsgemeinschaft (DFG, grants MU3133/3-1 and SFB1101).

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  1. Dorothee Stöckle

    Present address: †Present address: The Sainsbury Laboratory, University of Cambridge, Bateman Street, Cambridge CB2 1LR, UK.,

Authors and Affiliations

  1. Developmental Genetics, Centre for Plant Molecular Biology (ZMBP), University of Tübingen, Auf der Morgenstelle 32, 72076 Tübingen, Germany

    Dorothee Stöckle, Arvid Herrmann, Elisabeth Lipka, Theresa Lauster, Richard Gavidia, Steffi Zimmermann & Sabine Müller

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Contributions

We describe contributions to the paper using the CRediT taxonomy. Writing—original draft, S.M., D.S., A.H.; writing—review and editing: S.M.; conceptualization: D.S., S.M.; investigation: D.S., A.H., E.L., T.L.; methodology: S.M.; formal analysis: D.S., A.H., E.L. and S.M.; funding acquisition: S.M.

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Correspondence to Sabine Müller.

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The authors declare no competing financial interests.

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Supplementary Information

Supplementary Information, Supplementary Table 1, Supplementary References, Supplementary Figures 1–4. (PDF 2531 kb)

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Stöckle, D., Herrmann, A., Lipka, E. et al. Putative RopGAPs impact division plane selection and interact with kinesin-12 POK1. Nature Plants 2, 16120 (2016). https://doi.org/10.1038/nplants.2016.120

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