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The wheat Sr50 gene reveals rich diversity at a cereal disease resistance locus

Nature Plants volume 1, Article number: 15186 (2015) Cite this article

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Abstract

We identify the wheat stem rust resistance gene Sr50 (using physical mapping, mutation and complementation) as homologous to barley Mla, encoding a coiled-coil nucleotide-binding leucine-rich repeat (CC-NB-LRR) protein. We show that Sr50 confers a unique resistance specificity different from Sr31 and other genes on rye chromosome 1RS, and is effective against the broadly virulent Ug99 race lineage. Extensive haplotype diversity at the rye Sr50 locus holds promise for mining effective resistance genes.

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Figure 1: Sr50 locus and mutants.
Figure 2: Sr50 transgenic lines express resistance to stem rust.

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References

  1. Ellis, J. G., Lagudah, E. S., Spielmeyer, W. & Dodds, P. N. Front. Plant Sci. 5, 641 (2014).

    Article  Google Scholar 

  2. Singh, R. P. et al. Annu. Rev. Phytopathol. 49, 465–481 (2011).

    Article  CAS  Google Scholar 

  3. Zeller, F. J. in Proc. 4th International Wheat Genetics Symposium (eds Sears, E. R. & Sears, L. M. S. ) 209–221 (Univ. Missouri, 1973).

    Google Scholar 

  4. Shepherd, K. W. in Proc. 4th International Wheat Genetics Symposium (eds Sears, E. R. & Sears, L. M. S. ) 745–760 (Univ. Missouri, 1973).

    Google Scholar 

  5. Mago, R., Spielmeyer, W., Lawrence, G. J., Ellis, J. G. & Pryor, A. Genome 47, 112–121 (2004).

    Article  CAS  Google Scholar 

  6. Zeller, F. J. & Fuchs, E. Z. Z PflZücht 90, 285–296 (1983).

    Google Scholar 

  7. Jin, Y. & Singh, R. P. Plant Dis. 90, 476–480 (2006).

    Article  CAS  Google Scholar 

  8. Mago, R. et al. Theor. Appl. Genet. 112, 41–50 (2005).

    Article  CAS  Google Scholar 

  9. Wei, F. et al. Genetics 153, 1929–1948 (1999).

    CAS  PubMed  PubMed Central  Google Scholar 

  10. Šimková, H. et al. BMC Genomics 9, 237 (2008).

    Article  Google Scholar 

  11. Seeholzer, S. et al. Mol. Plant Microbe Int. 23, 497–509 (2010).

    Article  CAS  Google Scholar 

  12. Jordan, T. et al. Plant J. 65, 610–621 (2011).

    Article  CAS  Google Scholar 

  13. Periyannan, S. et al. Science 341, 786–788 (2013).

    Article  CAS  Google Scholar 

  14. Upadhyaya, N. et al. Front. Plant Sci. 5, 759 (2015).

    Article  Google Scholar 

  15. McIntosh, R. A., Wellings, C. R. & Park, R. F. Wheat Rusts: An Atlas of Resistance Genes (CSIRO Publishing, 1995).

    Book  Google Scholar 

  16. Sinnett, D., Richer, C. & Baccichet, A. BioTechniques 24, 752–754 (1998).

    Article  CAS  Google Scholar 

  17. Luo, M.-C. et al. Genomics 82, 378–389 (2003).

    Article  CAS  Google Scholar 

  18. Wang, M.-B., Matthews, P. R., Upadhyaya, N. M. & Waterhouse, P. M. Acta Hortic. 461, 401–405 (1998).

    Article  CAS  Google Scholar 

  19. Maas, C. et al. Mol. Breeding 3, 15–28 (1997).

    Article  CAS  Google Scholar 

  20. Richardson, T., Thistleton, J., Higgins, T. J., Howitt, C. & Ayliffe, M. Plant Cell Tis. Org. Culture 119, 647–659 (2014).

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This work was supported by funds provided through Grains Research and Development Corporation (CSP00161 and US00063). H.Š. and J.D. are supported by the National Program of Sustainability I (award LO1204). We are grateful to T. Richardson, D. Bhatt, S. Louis, X. Xia (CSIRO Agriculture, Australia) H. Miah and S. Hoxha (The University of Sydney, Australia) for technical assistance.

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Authors and Affiliations

  1. CSIRO Agriculture, GPO Box 1600, Canberra, ACT 2601, Australia

    Rohit Mago, Sambasivam Periyannan, Michael A. Ayliffe, Wolfgang Spielmeyer, Evans S. Lagudah, Jeff G. Ellis & Peter N. Dodds

  2. Plant Breeding Institute, The University of Sydney, Private Bag 4011, Narellan, 2567, New South Wales, Australia

    Peng Zhang, Urmil Bansal, Haydar Karaoglu, Harbans Bariana, Robert F. Park & Robert McIntosh

  3. INRA – CNRGV, 24 Chemin de Borde Rouge – Auzeville, CS 52627, Castanet Tolosan Cedex, 31326, France

    Sonia Vautrin & Hélène Bergès

  4. Institute of Experimental Botany, Centre of the Region Haná for Biotechnological and Agricultural Research, Šlechtitelů 31, Olomouc, CZ-78371, Czech Republic

    Hana Šimková & Jaroslav Doležel

  5. Department of Plant Sciences, University of California, Davis, 95616, California, USA

    Ming-Cheng Luo

  6. USDA, ARS Cereal Disease Laboratory, University of Minnesota, Saint Paul, 55108, Minnesota, USA

    Matthew Rouse, James Kolmer & Yue Jin

Authors
  1. Rohit Mago
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  2. Peng Zhang
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  3. Sonia Vautrin
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  4. Hana Šimková
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  5. Urmil Bansal
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  6. Ming-Cheng Luo
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  7. Matthew Rouse
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  8. Haydar Karaoglu
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  9. Sambasivam Periyannan
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  10. James Kolmer
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  11. Yue Jin
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  12. Michael A. Ayliffe
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  13. Harbans Bariana
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  14. Robert F. Park
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  15. Robert McIntosh
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  16. Jaroslav Doležel
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  17. Hélène Bergès
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  18. Wolfgang Spielmeyer
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  19. Evans S. Lagudah
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  20. Jeff G. Ellis
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  21. Peter N. Dodds
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Contributions

R.M., J.E., P.D. and E.L. conceived and planned the work. R.M., P.Z., S.V., H.S., U.B., M.-C.L., M.R., H.K., S.P., J.K., Y.J., M.A., H.S.B., R.F.P., R.McI., J.D. and H.B. performed the experiments and analysed the data. R.M. and P.D. wrote the manuscript and all co-authors commented on the draft.

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Correspondence to Peter N. Dodds.

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The authors declare no competing financial interests.

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Mago, R., Zhang, P., Vautrin, S. et al. The wheat Sr50 gene reveals rich diversity at a cereal disease resistance locus. Nature Plants 1, 15186 (2015). https://doi.org/10.1038/nplants.2015.186

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