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Single-cell biological lasers

Nature Photonics 5, 406–410 (2011) | Download Citation

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Abstract

Since their invention some 50 years ago1, lasers have made a tremendous impact on modern science and technology. Nevertheless, lasing has so far relied on artificial or engineered optical gain materials, such as doped crystals, semiconductors, synthetic dyes and purified gases2,3. Here, we show that fluorescent proteins4,5 in cells are a viable gain medium for optical amplification, and report the first successful realization of biological cell lasers based on green fluorescent protein (GFP). We demonstrate in vitro protein lasers using recombinant GFP solutions and introduce a laser based on single live cells expressing GFP. On optical pumping with nanojoule/nanosecond pulses, individual cells in a high-Q microcavity produce bright, directional and narrowband laser emission, with characteristic longitudinal and transverse modes. Lasing cells remained alive even after prolonged lasing action. Light amplification and lasing from and within biological systems pave the way to new forms of intracellular sensing, cytometry and imaging.

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Acknowledgements

The authors thank Ji-Joon Song (KAIST, Korea) for providing recombinant eGFP solutions, S. Sassi and B. Seed (Harvard Medical School) for the donation of 293ETN cells and support with eGFP transfection, U. Shama for initial testing of a fluorescent protein and W. Farinelli for setting up the OPO system. This work was supported in part by the US National Science Foundation (ECCS-1101947) and the Korea National Research Foundation (R31-2008-000-10071-0). M.C.G. acknowledges financial support from the Bullock-Wellman Fellowship.

Author information

Affiliations

  1. Wellman Center for Photomedicine, Massachusetts General Hospital, 55 Fruit Street, Boston, Massachusetts 02114, USA

    • Malte C. Gather
    •  & Seok Hyun Yun

  2. Department of Dermatology, Harvard Medical School, 55 Fruit Street, Boston, Massachusetts 02114, USA

    • Malte C. Gather
    •  & Seok Hyun Yun

  3. WCU Graduate School of Nanoscience and Technology, Korea Advanced Institute of Science and Technology, Daejeon, Korea

    • Seok Hyun Yun

  4. Harvard–MIT Health Sciences and Technology, Cambridge, Massachusetts, USA

    • Seok Hyun Yun

Authors

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Contributions

M.C.G. designed and performed the experiments. S.H.Y conceived and supervised the project. Both authors discussed the data and wrote the paper.

Competing interests

The authors declare no competing financial interests.

Corresponding author

Correspondence to Seok Hyun Yun.

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DOI

https://doi.org/10.1038/nphoton.2011.99