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Large-area spatially ordered arrays of gold nanoparticles directed by lithographically confined DNA origami

Nature Nanotechnology volume 5, pages 121–126 (2010)Cite this article

Abstract

The development of nanoscale electronic and photonic devices will require a combination of the high throughput of lithographic patterning and the high resolution and chemical precision afforded by self-assembly1,2,3,4. However, the incorporation of nanomaterials with dimensions of less than 10 nm into functional devices has been hindered by the disparity between their size and the 100 nm feature sizes that can be routinely generated by lithography. Biomolecules offer a bridge between the two size regimes, with sub-10 nm dimensions, synthetic flexibility and a capability for self-recognition. Here, we report the directed assembly of 5-nm gold particles into large-area, spatially ordered, two-dimensional arrays through the site-selective deposition of mesoscopic DNA origami5 onto lithographically patterned substrates6 and the precise binding of gold nanocrystals to each DNA structure. We show organization with registry both within an individual DNA template and between components on neighbouring DNA origami, expanding the generality of this method towards many types of patterns and sizes.

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Figure 1: Process for assembling two-dimensional nanoparticle arrays.
Figure 2: Binding of DNA origami to a surface.
Figure 3: Binding efficiencies of 5-nm gold nanoparticles to corners of A30-modified triangular DNA origami either in solution or adsorbed on oxide substrates.
Figure 4: Two-dimensional arrays of gold nanoparticles directed by lithographically confined DNA origami.
Figure 5: Geometrically packed arrays of multiple DNA origami conjugated with gold nanocrystals.

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Acknowledgements

This work was financially supported by the Center on Polymer Interfaces and Macromolecular Assemblies (award no. NSF DMR 0213618), the Office of Naval Research (award no. N00014-09-01-0250) and UCSD startup funds. The authors thank B. Davis for optical lithography, P.W.K. Rothemund for helpful discussions and comments on the manuscript, and S. Swanson, C. Rettner and M. Sanchez for helpful discussions.

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Authors and Affiliations

  1. Department of Nanoengineering, 9500 Gilman Drive M/C 0448

    Albert M. Hung & Jennifer N. Cha

  2. University of California San Diego, La Jolla, 92093-0448, California, USA

    Albert M. Hung & Jennifer N. Cha

  3. IBM Almaden Research Center, 650 Harry Road, San Jose, 95120, California, USA

    Albert M. Hung, Christine M. Micheel, Luisa D. Bozano, Lucas W. Osterbur, Greg M. Wallraff & Jennifer N. Cha

  4. The National Academies, Washington, DC, 20001, USA

    Christine M. Micheel

  5. Department of Materials Science and Engineering, University of Illinois at Urbana-Champaign, Urbana, 61801, Illinois, USA

    Lucas W. Osterbur

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  1. Albert M. Hung
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Contributions

A.M.H., C.M.M. and J.N.C. conceived and designed the experiments. A.M.H., C.M.M. and L.W.O. performed the experiments. L.D.B. contributed the lithographically patterned substrates. A.M.H. and J.N.C. analysed the data. A.M.H. and J.N.C. co-wrote the paper. All authors discussed the results and commented on the manuscript.

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Correspondence to Jennifer N. Cha.

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Hung, A., Micheel, C., Bozano, L. et al. Large-area spatially ordered arrays of gold nanoparticles directed by lithographically confined DNA origami. Nature Nanotech 5, 121–126 (2010). https://doi.org/10.1038/nnano.2009.450

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