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Synaptic vesicles recycling spontaneously and during activity belong to the same vesicle pool

Nature Neuroscience volume 10, pages 145–147 (2007)Cite this article

Abstract

Recently, it has been claimed that vesicles recycling spontaneously and during activity belong to different pools. Here we simultaneously measured, using spectrally separable styryl dyes, the release kinetics of vesicles recycled spontaneously or upon stimulation and the effects of the v-ATPase blocker folimycin on the frequency of miniature postsynaptic currents in rat hippocampal neurons. Our results provide evidence as to the identities of the vesicle pools recycling at rest and during stimulation.

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Figure 1: Simultaneous measurement of release kinetics of synaptic vesicles stained by styryl dyes both spontaneously and during stimulation.
Figure 2: Release kinetics and fluorescence amplitudes are highly correlated for spontaneous and activity-dependent recycling at individual boutons, but vary highly between boutons.
Figure 3: Folimycin decreases the frequency of spontaneous release events in an activity-dependent manner.

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Acknowledgements

We are grateful to H. Taschenberger for extensive help with miniature analysis, M. Wienisch for advice in imaging analysis and E. Neher for critical reading of the manuscript. We thank all laboratory members for their support, M. Pilot for expert technical assistance and S. Gliem and T. Frank for participating in some of the initial experiments. This work was supported by a grant from the Human Frontier Science Project (J.K).

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Authors and Affiliations

  1. Department of Membrane Biophysics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, Goettingen, 37077, Germany

    Teja W Groemer & Jurgen Klingauf

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  1. Teja W Groemer
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  2. Jurgen Klingauf
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Correspondence to Jurgen Klingauf.

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The authors declare no competing financial interests.

Supplementary information

Supplementary Fig. 1

Normalization of data with poor signal-to-noise introduces artifacts. (PDF 73 kb)

Supplementary Fig. 2

Folimycin decreases mEPSC's at 37°C. (PDF 38 kb)

Supplementary Discussion (PDF 20 kb)

Supplementary Methods (PDF 93 kb)

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Groemer, T., Klingauf, J. Synaptic vesicles recycling spontaneously and during activity belong to the same vesicle pool. Nat Neurosci 10, 145–147 (2007). https://doi.org/10.1038/nn1831

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