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Light-activated ion channels for remote control of neuronal firing

Nature Neuroscience volume 7, pages 13811386 (2004) | Download Citation

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Abstract

Neurons have ion channels that are directly gated by voltage, ligands and temperature but not by light. Using structure-based design, we have developed a new chemical gate that confers light sensitivity to an ion channel. The gate includes a functional group for selective conjugation to an engineered K+ channel, a pore blocker and a photoisomerizable azobenzene. Long-wavelength light drives the azobenzene moiety into its extended trans configuration, allowing the blocker to reach the pore. Short-wavelength light generates the shorter cis configuration, retracting the blocker and allowing conduction. Exogenous expression of these channels in rat hippocampal neurons, followed by chemical modification with the photoswitchable gate, enables different wavelengths of light to switch action potential firing on and off. These synthetic photoisomerizable azobenzene-regulated K+ (SPARK) channels allow rapid, precise and reversible control over neuronal firing, with potential applications for dissecting neural circuits and controlling activity downstream from sites of neural damage or degeneration.

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Acknowledgements

We thank S. Ahituv for technical support, R. Fredrick and J. Harvey for contributing to the chemical synthesis, I. Hafez and C. Nam for help with cell culture, F. Tombola for advice and C. Luetje for comments on the manuscript. This work was supported by a Fight-for-Sight grant to R.H.K. and funds from the Lawrence Berkeley National Laboratory to D.T. K.B. was supported by a Howard Hughes Medical Institute predoctoral fellowship.

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Author notes

    • Matthew Banghart
    •  & Katharine Borges

    These authors contributed equally to this work.

Affiliations

  1. Department of Chemistry, University of California, Berkeley, California 94720, USA.

    • Matthew Banghart
    •  & Dirk Trauner
  2. Department of Molecular and Cell Biology, University of California, Berkeley, California 94720, USA.

    • Katharine Borges
    • , Ehud Isacoff
    •  & Richard H Kramer

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The authors declare no competing financial interests.

Corresponding authors

Correspondence to Dirk Trauner or Richard H Kramer.

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DOI

https://doi.org/10.1038/nn1356