Abstract
The zebrafish dorsal habenula (dHb) shows conspicuous asymmetry in its connection with the interpeduncular nucleus (IPN) and is equivalent to the mammalian medial habenula. Genetic inactivation of the lateral subnucleus of dHb (dHbL) biased fish towards freezing rather than the normal flight response to a conditioned fear stimulus, suggesting that the dHbL-IPN pathway is important for controlling experience-dependent modification of fear responses.
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Change history
17 October 2010
In the version of this article initially published online, there was an error on page 2, right column, second paragraph, 14th line. Here, ‘Mtz’ should read ‘metronidazole’. The supplementary material file was also missing some information from the images. Both errors have been corrected for the print, PDF and HTML versions of this article.
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Acknowledgements
We thank Y. Yoshihara, T. Koide and other members of the Yoshihara laboratory for transgenic fish and advice on the behavioral experiments, S. Watanabe for help in setting up the behavioral experiments, N. Copeland for the BAC homologous recombination system, S. Fraser for GAL4VP16 and UAS plasmids, M. Parsons for the UAS:nfsB-mCherry plasmid, A. Thomson for correction of the manuscript and S. Jesuthasan for communication of unpublished data. We are grateful to all of the members of the Okamoto laboratory for support and advice, the Research Resource Center of RIKEN BSI for animal care and the National BioResource Project of Japan for fish strains. This work was supported by Grant-in-Aids from the Ministry of Education, Culture, Sports, Science and Technology of Japan to H.O. and H.A. and by the Core Research for Evolutional Science and Technology of Japan Science and Technology Agency to H.O.
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M.A., H.A. and H.O. designed the experiments and wrote the manuscript. H.O. supervised the research project. M.A. performed most of the experiments using transgenic fish with T.A., R.N., M.T., T. Sassa, T. Shiraki, K.K., T.H. and S.H. H.A. performed the neural tracing study with M.G., M.T. and R.A.
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Supplementary Text and Figures
Supplementary Figures 1–24, Supplementary Table 1, Supplementary Methods and Supplementary Data (PDF 4168 kb)
Supplementary Movie 1
Response of the control fish at the first trial of the retrieval session. (MOV 494 kb)
Supplementary Movie 2
Response of the dHbl-silenced fish at the first trial of the retrieval session. (MOV 1138 kb)
Supplementary Movie 3
Persistent rotation of the dHbl-silenced fish at the first trial of the retrieval session. (MOV 581 kb)
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Agetsuma, M., Aizawa, H., Aoki, T. et al. The habenula is crucial for experience-dependent modification of fear responses in zebrafish. Nat Neurosci 13, 1354–1356 (2010). https://doi.org/10.1038/nn.2654
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DOI: https://doi.org/10.1038/nn.2654
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