Carbapenem and colistin are the last-resort antibiotics used for treating multidrug-resistant Gram-negative pathogens. Here, we report, for the first time, co-transfer of resistance to both classes of antibiotics by a mobile IncX3–X4 hybrid plasmid in an Escherichia coli isolate. Spread of such a plasmid is of great concern for clinical therapy, and heightened efforts are needed to control its dissemination.
This work was supported by the National Natural Science Foundation and Natural Science Foundation of Guangdong Province, China (U1201214), the Program for Changjiang Scholars and Innovative Research Team in University of Ministry of Education of China (IRT13063), the Natural Science Foundation of Guangdong Province (S2012030006590) and the National Basic Research Program of China (2016YFC1200100).
Supplementary Figures 1-3, Supplementary Tables 1-2