Brief Communication

Co-transfer of blaNDM-5 and mcr-1 by an IncX3–X4 hybrid plasmid in Escherichia coli

  • Nature Microbiology 1, Article number: 16176 (2016)
  • doi:10.1038/nmicrobiol.2016.176
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Abstract

Carbapenem and colistin are the last-resort antibiotics used for treating multidrug-resistant Gram-negative pathogens. Here, we report, for the first time, co-transfer of resistance to both classes of antibiotics by a mobile IncX3–X4 hybrid plasmid in an Escherichia coli isolate. Spread of such a plasmid is of great concern for clinical therapy, and heightened efforts are needed to control its dissemination.

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Acknowledgements

This work was supported by the National Natural Science Foundation and Natural Science Foundation of Guangdong Province, China (U1201214), the Program for Changjiang Scholars and Innovative Research Team in University of Ministry of Education of China (IRT13063), the Natural Science Foundation of Guangdong Province (S2012030006590) and the National Basic Research Program of China (2016YFC1200100).

Author information

Author notes

    • Jian Sun
    •  & Run-Shi Yang

    These authors contributed equally to this work.

Affiliations

  1. National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, South China Agricultural University, Guangzhou 510642, China

    • Jian Sun
    • , Run-Shi Yang
    • , Liang-Xing Fang
    • , Jing Xia
    • , Liang Li
    • , Xiao-Yue Lv
    • , Jia-Hong Duan
    • , Xiao-Ping Liao
    •  & Ya-Hong Liu
  2. College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China

    • Jian Sun
    • , Run-Shi Yang
    • , Liang-Xing Fang
    • , Jing Xia
    • , Liang Li
    • , Xiao-Yue Lv
    • , Jia-Hong Duan
    • , Xiao-Ping Liao
    •  & Ya-Hong Liu
  3. Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa 50011, USA

    • Qijing Zhang
  4. Department of Medical Microbiology and Parasitology, Zhejiang University School of Medicine, Zhejiang 310027, China

    • Youjun Feng
  5. Jiangsu Co-Innovation Centre for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu 225009, China

    • Ya-Hong Liu

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Contributions

J.S., R.-S.Y., Q.Z., Y.F., X.-P.L. and Y.-H.L. designed the study and prepared the manuscript. R.-S.Y., L.-X.F., J.X., L.L., X.-Y.L. and J.-H.D. performed experiments. All authors discussed the results. J.S., R.-S.Y., Q.Z., Y.F., X.L. and Y.-H.L. analysed data. J.S., Q.Z., Y.F. and Y.-H.L. drafted this manuscript.

Competing interests

The authors declare no competing financial interests.

Corresponding authors

Correspondence to Xiao-Ping Liao or Ya-Hong Liu.

Supplementary information

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    Supplementary information

    Supplementary Figures 1-3, Supplementary Tables 1-2