Analysis

Metagenomics uncovers gaps in amplicon-based detection of microbial diversity

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Abstract

Our view of microbial diversity has expanded greatly over the past 40 years, primarily through the wide application of PCR-based surveys of the small-subunit ribosomal RNA (SSU rRNA) gene. Yet significant gaps in knowledge remain due to well-recognized limitations of this method. Here, we systematically survey primer fidelity in SSU rRNA gene sequences recovered from over 6,000 assembled metagenomes sampled globally. Our findings show that approximately 10% of environmental microbial sequences might be missed from classical PCR-based SSU rRNA gene surveys, mostly members of the Candidate Phyla Radiation (CPR) and as yet uncharacterized Archaea. These results underscore the extent of uncharacterized microbial diversity and provide fruitful avenues for describing additional phylogenetic lineages.

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Author information

Affiliations

  1. Joint Genome Institute, Walnut Creek, California 94598, USA

    • Emiley A. Eloe-Fadrosh
    • , Natalia N. Ivanova
    • , Tanja Woyke
    •  & Nikos C. Kyrpides

Authors

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Contributions

E.A.E.-F., N.N.I., T.W. and N.C.K. designed the project, analysed the data and wrote the manuscript. All authors discussed the results and commented on the manuscript.

Competing interests

The authors declare no competing financial interests.

Corresponding author

Correspondence to Nikos C. Kyrpides.

Supplementary information

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    Supplementary Information

    Supplementary Figures 1–3, Supplementary Tables 1,2.