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Nanoscale resolution in GFP-based microscopy

Nature Methods 3, 721–723 (2006) | Download Citation

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Abstract

We report attainment of subdiffraction resolution using stimulated emission depletion (STED) microscopy with GFP-labeled samples. The ∼70 nm lateral resolution attained in this study is demonstrated by imaging GFP-labeled viruses and the endoplasmic reticulum (ER) of a mammalian cell. Our results mark the advent of nanoscale biological microscopy with genetically encoded markers.

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Acknowledgements

Purified GFP-VLPs were provided by J. Cohen and the plasmid eGFP-ER by P. Lipp. We thank S. Verrier, T. Rosenmund, A.C. Schauss, J.J. Sieber and T. Müller for providing samples, A. Schönle for help with the software ImSpector, V. Westphal and B. Harke for valuable discussions, as well as J. Keller and B. Rankin for critical reading. We thank R.Y. Tsien for providing the plasmids coding for mCitrine, mStrawbery and mRFP.

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Affiliations

  1. Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, Am Fassberg 11, 37077 Göttingen, Germany.

    • Katrin I Willig
    • , Robert R Kellner
    • , Rebecca Medda
    • , Birka Hein
    • , Stefan Jakobs
    •  & Stefan W Hell

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Competing interests

The authors declare no competing financial interests.

Corresponding author

Correspondence to Stefan W Hell.

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DOI

https://doi.org/10.1038/nmeth922