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Single protein complex visualization: Seeing is believing

Protein-protein interactions are at the heart of the cellular machinery. Direct in-cell visualization of single, endogenous protein interaction pairs now becomes possible.

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Figure 1: FRET, BiFC and P-LISA each allow the in-cell visualization of protein complexes through monitoring fluorescence.

References

  1. Wallrabe, H. & Periasamy, A. Curr. Opin. Biotechnol. 16, 19–27 (2005).

    Article  CAS  Google Scholar 

  2. Kerppola, T.K. Nat.Rev. Mol. Cell. Biol. 7, 449–456 (2006).

    Article  CAS  Google Scholar 

  3. Eyckerman, S. et al. Nat. Cell. Biol. 3, 1114–1119 (2001).

    Article  CAS  Google Scholar 

  4. Söderberg, O. et al. Nat. Methods 3, 995–1000 (2006).

    Article  Google Scholar 

  5. Fredriksson, S. et al. Nat. Biotechnol. 20, 473–477 (2002).

    Article  CAS  Google Scholar 

  6. Eyckerman, S. et al. Nat. Methods 2, 427–433 (2005).

    Article  CAS  Google Scholar 

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Lievens, S., Tavernier, J. Single protein complex visualization: Seeing is believing. Nat Methods 3, 971–972 (2006). https://doi.org/10.1038/nmeth1206-971

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