Abstract
We developed a rapid, economical method for high-resolution quantitative trait locus (QTL) mapping using microarrays for selective genotyping of pooled DNA samples. We generated 21,207 F2 flies from two inbred Drosophila melanogaster strains with known QTLs affecting lifespan, and hybridized DNA pools of young and old flies to microarrays. We used changes of gene frequency of 2,326 single-feature polymorphisms (SFPs) to map previously identified and additional QTLs affecting lifespan.
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Acknowledgements
We thank R. Wilson for help with the fly work. This work was supported by contracts 53-K06-5-10 and 58-1950-9-001 from the US Department of Agriculture's Agriculture Research Service and by National Institutes of Health grants GM45344 and GM45146.
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A patent application associated with techniques used in this article is being prepared.
W.Z. is an employee of PGxHealth.
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Supplementary Text and Figures
Supplementary Figure 1–3, Supplementary Tables 1, 3–5, Supplementary Methods (PDF 322 kb)
Supplementary Table 2
Positional candidate genes within QTL regions. (XLS 121 kb)
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Lai, CQ., Leips, J., Zou, W. et al. Speed-mapping quantitative trait loci using microarrays. Nat Methods 4, 839–841 (2007). https://doi.org/10.1038/nmeth1084
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DOI: https://doi.org/10.1038/nmeth1084