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Use of quantum dots for live cell imaging

Nature Methods volume 1, pages 73–78 (2004)Cite this article

  • Quantum dots (QDs; e.g., Quantum Dot Corporation or Evident Technology)

  • Cells or tissue for labeling, prepared appropriately depending on the application (QDs can be used to tag live cells, label cell-surface proteins, or label fixed cells or tissue sections)

  • Amylose resin (New England Biolabs)

  • Antibodies of interest

  • Avidin (Sigma Chemicals)

  • Bovine serum albumin (BSA), 1% in PBS

  • Lipid-based transfection reagent (e.g., Lipofectamine 2000 (Invitrogen) or Fugene 6 (Roche))

  • Maltose (Sigma Chemicals)

  • Maltose-binding protein fused to the basic leucine zipper domain (MBP-zb) and protein G fused to the basic leucine zipper domain (PG-zb) expressed and purified from bacteria as described elsewhere7.

  • Phosphate-buffered saline (PBS; Sigma Chemicals)

  • Sodium tetraborate (Sigma Chemicals)

  • Sulfo-NHS-SS biotin (Pierce Biotechnology)

  • Tris-buffered saline (TBS; Sigma Chemicals)

Quantum dots (QDs; e.g., Quantum Dot Corporation or Evident Technology)

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Figure 1: The mixed surface approach for conjugating biomolecules to DHLA-capped QDs using avidin as the linker molecule.
Figure 2: Distribution of GM1 gangliosides found in the plasma membrane.

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Author information

Authors and Affiliations

  1. The Rockefeller University, Laboratory of Cellular Biophysics, 1230 York Avenue, New York, 10021, New York, USA

    Jyoti K Jaiswal & Sanford M Simon

  2. Center for Bio/Molecular Science and Engineering, United States Naval Research Laboratory, Washington, 20375, DC, USA

    Ellen R Goldman

  3. Division of Optical Sciences, United States Naval Research Laboratory, Washington, 20375, DC, USA

    Hedi Mattoussi

Authors
  1. Jyoti K Jaiswal
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  2. Ellen R Goldman
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  3. Hedi Mattoussi
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  4. Sanford M Simon
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Jaiswal, J., Goldman, E., Mattoussi, H. et al. Use of quantum dots for live cell imaging. Nat Methods 1, 73–78 (2004). https://doi.org/10.1038/nmeth1004-73

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