An approach using multiple fluorochrome combinations allows the simultaneous detection of many T-cell populations within a single blood sample.
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Kedzierska, K., Stambas, J. & Doherty, P. Finding multiple needles in one immune haystack. Nat Methods 6, 489–490 (2009). https://doi.org/10.1038/nmeth0709-489
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DOI: https://doi.org/10.1038/nmeth0709-489