Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Correspondence
  • Published:

Unexpected failure rates for modular assembly of engineered zinc fingers

Nature Methods volume 5, pages 374–375 (2008)Cite this article

An Erratum to this article was published on 01 June 2008

This article has been updated

NOTE: In the version of this correspondence initially published, the two previously published datasets analyzed were labeled with incorrect references in Figure 1b. Reference 2 should be associated with the second column (80 sites) and Reference 3 should be associated with the third column (96 sites). The weblink for the Zinc Finger Consortium website was incorrect and should be http://www.zincfingers.org/. The errors have been corrected in the HTML and PDF versions of the article.

To the editor:

This is a preview of subscription content, access via your institution

Relevant articles

Open Access articles citing this article.

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Figure 1: Large-scale evaluation of the modular assembly method for engineering zinc-finger arrays.

Change history

  • 29 May 2008

    In the version of this correspondence initially published, the two previously published datasets analyzed were labeled with incorrect references in Figure 1b. Reference 2 should be associated with the second column (80 sites) and Reference 3 should be associated with the third column (96 sites). The weblink for the Zinc Finger Consortium website was incorrect and should be http://www.zincfingers.org/. The errors have been corrected in the HTML and PDF versions of the article.

References

  1. Porteus, M.H. & Carroll, D. Nat. Biotechnol. 23, 967–973 (2005).

    Article  CAS  Google Scholar 

  2. Segal, D.J. et al. Biochemistry 42, 2137–2148 (2003).

    Article  CAS  Google Scholar 

  3. Bae, K.H. et al. Nat. Biotechnol. 21, 275–280 (2003).

    Article  CAS  Google Scholar 

  4. Mandell, J.G. & Barbas, C.F. Nucleic Acids Res. 34, W516–W523 (2006).

    Article  CAS  Google Scholar 

  5. Liu, Q., Xia, Z., Zhong, X. & Case, C.C. J. Biol. Chem. 277, 3850–3856 (2002).

    Article  CAS  Google Scholar 

  6. Wright, D.A. et al. Nat. Protoc. 1, 1637–1652 (2006).

    Article  Google Scholar 

Download references

Acknowledgements

We thank D. Dobbs and P. Zaback for helpful discussions and E. Unger-Wallace (Iowa State University) for contributions to zinc-finger array construction. J.K.J. is supported by the US National Institutes of Health (R21 RR024189), the Cystic Fibrosis Foundation (MCCRAY07G0) and the Massachusetts General Hospital Department of Pathology; D.F.V. is supported by a US National Science Foundation grant DBI 0501678; T.C. is supported by the German Research Foundation (SPP1230 CA311/2); C.L.R. is supported by a Ford Foundation Pre-Doctoral Diversity Fellowship and a National Science Foundation Graduate Research Fellowship; T.I.C. is supported by a fellowship from the Swiss Foundation for Grants in Biology and Medicine.

Author information

Author notes

  1. Cherie L Ramirez and Jonathan E Foley: These authors contributed equally to this work.

Authors and Affiliations

  1. Molecular Pathology Unit, Center for Cancer Research, and Center for Computational and Integrative Biology, 149 13th Street, Room 7132, Massachusetts General Hospital, Charlestown, 02129, Massachusetts, USA

    Cherie L Ramirez, Jonathan E Foley & J Keith Joung

  2. Division of Medical Sciences, Biological and Biomedical Sciences Program, Harvard Medical School, Boston, 02115, Massachusetts, USA

    Cherie L Ramirez & J Keith Joung

  3. Department of Genetics, Development and Cell Biology, Iowa State University, 1035A Roy J. Carver Co-Lab, Ames, 50011, Iowa, USA

    David A Wright, Ronnie J Winfrey, Jeffry D Sander, Fengli Fu, Jeffrey A Townsend & Daniel F Voytas

  4. Charité Medical School, Institute of Virology, Hindenburgdamm 27, Berlin, D-12203, Germany

    Felix Müller-Lerch, Shamim H Rahman, Tatjana I Cornu & Toni Cathomen

  5. Interdepartmental Graduate Program in Bioinformatics and Computational Biology, 2114 Molecular Biology Building, Iowa State University, Ames, 50011, Iowa, USA

    Jeffry D Sander & Fengli Fu

  6. Department of Pathology, Harvard Medical School, Boston, 02115, Massachusetts, USA

    J Keith Joung

Authors
  1. Cherie L Ramirez
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jonathan E Foley
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. David A Wright
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Felix Müller-Lerch
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Shamim H Rahman
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Tatjana I Cornu
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Ronnie J Winfrey
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Jeffry D Sander
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Fengli Fu
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Jeffrey A Townsend
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. Toni Cathomen
    View author publications

    You can also search for this author in PubMed Google Scholar

  12. Daniel F Voytas
    View author publications

    You can also search for this author in PubMed Google Scholar

  13. J Keith Joung
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to J Keith Joung.

Supplementary information

Supplementary Text and Figures

Supplementary Figures 1–4, Supplementary Tables 1–2, Supplementary Discussion, Supplementary Methods (PDF 319 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ramirez, C., Foley, J., Wright, D. et al. Unexpected failure rates for modular assembly of engineered zinc fingers. Nat Methods 5, 374–375 (2008). https://doi.org/10.1038/nmeth0508-374

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1038/nmeth0508-374

This article is cited by

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing