We present Omni-ATAC, an improved ATAC-seq protocol for chromatin accessibility profiling that works across multiple applications with substantial improvement of signal-to-background ratio and information content. The Omni-ATAC protocol generates chromatin accessibility profiles from archival frozen tissue samples and 50-μm sections, revealing the activities of disease-associated DNA elements in distinct human brain structures. The Omni-ATAC protocol enables the interrogation of personal regulomes in tissue context and translational studies.
Sequence Read Archive
Gene Expression Omnibus
We thank the Stanford Alzheimer's Disease Research Center (NIH P50 AG047366; to V. Henderson), the Pacific Udall Center for Excellence in Parkinson's Disease Research (NIH P50 NS062684; T.J.M.), and their participants for donating samples for research. We also thank J. Coller and X. Ji for sequencing assistance, E. Plowey and D. Channappa for tissue preparation, and P. Chu and A. Grewall for histology assistance. This work was supported by a grant from the Leukemia & Lymphoma Society Career Development Program (M.R.C.), US National Institutes of Health (NIH) training grant R25CA180993 (M.R.C.), NIH grants P50-HG007735 (H.Y.C. and W.J.G.), UM1HG00943 (W.J.G.), and U19AI057266 (W.J.G.), National Institute on aging grant RF1 AG053959 (T.J.M.), the Rita Allen Foundation (W.J.G.), the Human Frontier Science Program (W.J.G.), the National Science Foundation Graduate Research Fellowship Program (A.E.T.), and a US Department of Defense National Defense Science and Engineering Graduate (NDSEG) Fellowship (N.A.S.-A.).