Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • News & Views
  • Published:

Cas9 in action: no more known unknowns?

Useful new methods are being introduced to experimentally determine the genome-wide consequences of Cas9-based editing.

This is a preview of subscription content, access via your institution

Access options

Buy this article

Prices may be subject to local taxes which are calculated during checkout

Figure 1: Naked genomic DNA is treated with a purified nuclease (turquoise) targeted to a specific genomic region.

References

  1. Tsai, S.Q. et al. Nat. Methods 14, 607–614 (2017).

    Article  CAS  Google Scholar 

  2. Cameron, P. et al. Nat. Methods 14, 600–606 (2017).

    Article  CAS  Google Scholar 

  3. Carroll, D. Annu. Rev. Biochem. 83, 409–439 (2014).

    Article  CAS  Google Scholar 

  4. Tebas, P. et al. N. Engl. J. Med. 370, 901–910 (2014).

    Article  CAS  Google Scholar 

  5. Qasim, W. et al. Sci. Transl. Med. 9, eaaj2013 (2017).

    Article  Google Scholar 

  6. Bibikova, M., Golic, M., Golic, K.G. & Carroll, D. Genetics 161, 1169–1175 (2002).

    CAS  PubMed  PubMed Central  Google Scholar 

  7. Tsai, S.Q. et al. Nat. Biotechnol. 33, 187–197 (2015).

    Article  CAS  Google Scholar 

  8. Kim, D. et al. Nat. Methods 12, 237–243 (2015).

    Article  CAS  Google Scholar 

  9. Knight, S.C. et al. Science 350, 823–826 (2015).

    Article  CAS  Google Scholar 

  10. Urnov, F. Nature 529, 468–469 (2016).

    Article  CAS  Google Scholar 

  11. Shalem, O., Sanjana, N.E. & Zhang, F. Nat. Rev. Genet. 16, 299–311 (2015).

    Article  CAS  Google Scholar 

  12. Urnov, F.D. et al. Nature 435, 646–651 (2005).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Fyodor D Urnov.

Ethics declarations

Competing interests

The author declares no competing financial interests.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Urnov, F. Cas9 in action: no more known unknowns?. Nat Methods 14, 563–564 (2017). https://doi.org/10.1038/nmeth.4301

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/nmeth.4301

Search

Quick links

Nature Briefing: Translational Research

Sign up for the Nature Briefing: Translational Research newsletter — top stories in biotechnology, drug discovery and pharma.

Get what matters in translational research, free to your inbox weekly. Sign up for Nature Briefing: Translational Research