Two surveys of over 1,700 publications whose authors use quantitative real-time PCR (qPCR) reveal a lack of transparent and comprehensive reporting of essential technical information. Reporting standards are significantly improved in publications that cite the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines, although such publications are still vastly outnumbered by those that do not.
This is a preview of subscription content, access via your institution
Relevant articles
Open Access articles citing this article.
-
Development of quantitative multiplex RT-qPCR one step assay for detection of hepatitis delta virus
Scientific Reports Open Access 26 July 2023
-
Deciphering the sex bias in housekeeping gene expression in adipose tissue: a comprehensive meta-analysis of transcriptomic studies
Biology of Sex Differences Open Access 18 April 2023
-
Validation of reference genes for whole blood gene expression analysis in cord blood of preterm and full-term neonates and peripheral blood of healthy adults
BMC Genomics Open Access 30 June 2021
Access options
Subscribe to this journal
Receive 12 print issues and online access
$259.00 per year
only $21.58 per issue
Rent or buy this article
Prices vary by article type
from$1.95
to$39.95
Prices may be subject to local taxes which are calculated during checkout
References
Bustin, S.A. J. Mol. Endocrinol. 25, 169–193 (2000).
Kubista, M. et al. Mol. Aspects Med. 27, 95–125 (2006).
Bustin, S.A. et al. Clin. Chem. 55, 611–622 (2009).
Bustin, S.A. Methods 50, 217–226 (2010).
Garson, J.A. et al. AIDS Res. Hum. Retroviruses 25, 377–378 (2009).
Derveaux, S., Vandesompele, J. & Hellemans, J. Methods 50, 227–230 (2010).
Scholl, C. et al. Cell 137, 821–834 (2009).
Babij, C. et al. Cancer Res. 71, 5818–5826 (2011).
Ioannidis, J.P. & Khoury, M.J. Science 334, 1230–1232 (2011).
Anonymous. Nature 496, 398 (2013).
Pfaffl, M.W. Nucleic Acids Res. 29, e45 (2001).
Huggett, J., Dheda, K., Bustin, S. & Zumla, A. Genes Immun. 6, 279–284 (2005).
Vandesompele, J. et al. Genome Biol. 3, research0034 (2002).
Fleige, S. & Pfaffl, M.W. Mol. Aspects Med. 27, 126–139 (2006).
Vermeulen, J. et al. Nucleic Acids Res. 39, e63 (2011).
Bustin, S.A. Expert Rev. Mol. Diagn. 5, 493–498 (2005).
Tricarico, C. et al. Anal. Biochem. 309, 293–300 (2002).
Bustin, S.A. et al. BMC Mol. Biol. 11, 74 (2010).
Bustin, S. & Penning, L.C. Vet. J. 191, 279–281 (2012).
Bustin, S. Int. J. Mol. Sci. 14, 15878–15884 (2013).
Bustin, S.A. et al. Clin. Chem. 57, 919–921 (2011).
Isolani, M.E. et al. Eur. J. Pharmacol. 686, 1–7 (2012).
Huggett, J. & Bustin, S.A. Accredit. Qual. Assur. 16, 399–405 (2011).
Banobi, J.A., Branch, T.A. & Hilborn, R. Ecosphere 2, art37 (2011).
Horrobin, D.F. Nat. Rev. Drug Discov. 2, 151–154 (2003).
Acknowledgements
This publication is dedicated to the memory of the late Claudio Orlando, Department of Clinical Pathophysiology, University of Florence, Italy.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Competing interests
The authors declare no competing financial interests.
Supplementary information
Supplementary Figures, Tables and Notes
Supplementary Figures 1–8, Supplementary Tables 1–7 and Supplementary Notes 1 and 2 (PDF 504 kb)
Supplementary Data
Excel file, which has four worksheets: “2009 to 2011” containing the scores for the 2009/2011 survey, “2012 to 2013 non-MIQE” containing the scores from papers not citing the MIQE guidelines, “2012 to 2013 MIQE” containing the scores from papers citing the MIQE guidelines and “2012 to 2013 commercial” containing the scores from papers using commercial assays). (XLS 122 kb)
Rights and permissions
About this article
Cite this article
Bustin, S., Benes, V., Garson, J. et al. The need for transparency and good practices in the qPCR literature. Nat Methods 10, 1063–1067 (2013). https://doi.org/10.1038/nmeth.2697
Published:
Issue Date:
DOI: https://doi.org/10.1038/nmeth.2697
This article is cited by
-
Simultaneous Triplex Detection in a Single-Test-Line Lateral Flow Immunoassay Utilizing Distinct Nanoparticle Colorimetry
BioChip Journal (2024)
-
Deciphering the sex bias in housekeeping gene expression in adipose tissue: a comprehensive meta-analysis of transcriptomic studies
Biology of Sex Differences (2023)
-
Investigation of the machining behavior of unidirectional Alfa (Stipa tenacissima L.)/epoxy composite material
The International Journal of Advanced Manufacturing Technology (2023)
-
Hydrogel capsule-based digital quantitative polymerase chain reaction
Microchimica Acta (2023)
-
Development of quantitative multiplex RT-qPCR one step assay for detection of hepatitis delta virus
Scientific Reports (2023)