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Hematopoietic stem cell transplantation without irradiation

Nature Methods volume 6, pages 267–269 (2009)Cite this article

Abstract

Hematopoietic stem cell (HSC) transplantation is limited by histocompatibility barriers and by lack of space in bone marrow niches. These obstacles prevent in vivo analysis of histoincompatible mutant stem cells and of HSC functions in non-irradiated mice. By genetically combining immunodeficiency with impaired function of the growth factor receptor Kit in mice, we generated a 'universal' HSC recipient that efficiently accepts long-term histocompatible and histoincompatible HSCs without prior irradiation.

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Figure 1: Allogeneic donor HSC reconstitution, CFU-S formation and HSC mutant analysis in non-irradiated Rag2−/−γc−/−KitW/Wv mice.

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Acknowledgements

We thank I. Horak (Leibniz Institute of Molecular Pharmacology, Berlin) and H.J. Fehling (Institute for Immunology, Ulm University) for providing Bclaf1-deficient and Mll5-deficient mice, respectively, G. Terszowski for help with flow cytometry and data analysis, S. Schlenner for help with bone marrow transplantations, and T. Feyerabend, C. Läufle and members of the animal facility for careful maintenance of mouse lines. H.-R.R. thanks S. Takeda for the original discussions on Kit compound-mutant mice. This work was supported by the Landesstiftung Baden-Württemberg (21-655.023/104) to C.W. and by the German Research Foundation (754-2-2) to H.-R.R.

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Author notes

  1. Susanne Bartels

    Present address: Present address: Division of Vascular Oncology and Metastasis, German Cancer Research Center (DKFZ), Heidelberg, Germany.,

Authors and Affiliations

  1. Center for Regenerative Therapies Dresden (CRTD), Dresden University of Technology, Dresden, Germany

    Claudia Waskow

  2. Institute for Immunology, University of Ulm, Ulm, Germany

    Claudia Waskow, Vikas Madan, Susanne Bartels, Céline Costa & Hans-Reimer Rodewald

  3. Leibniz-Institute of Molecular Pharmacology (FMP), Berlin, Germany

    Rosel Blasig

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  1. Claudia Waskow
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  2. Vikas Madan
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  6. Hans-Reimer Rodewald
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Contributions

C.W. designed and performed research, analyzed data and wrote the paper. V.M. designed and performed research on Mll5−/− mutants. S.B. and C.C. performed research and R.B. provided crucial reagents. H.-R.R. designed research, analyzed data and wrote the paper.

Corresponding authors

Correspondence to Claudia Waskow or Hans-Reimer Rodewald.

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Supplementary Figures 1–4, Supplementary Table 1, Supplementary Results, Supplementary Discussion, Supplementary Methods (PDF 3813 kb)

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Waskow, C., Madan, V., Bartels, S. et al. Hematopoietic stem cell transplantation without irradiation. Nat Methods 6, 267–269 (2009). https://doi.org/10.1038/nmeth.1309

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