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Complementary hydropathy identifies a cellular prion protein receptor

Nature Medicine volume 3, pages 1376–1382 (1997)Cite this article

Abstract

Prions, the etiological agents for infectious degenerative encephalopathies, act by entering the cell and inducing conformational changes in PrPc (a normal cell membrane sialoglycoprotein), which result in cell death. A specific cell-surface receptor to mediate PrPc and prion endocytosis has been predicted. Complementary hydropathy let us generate a hypothetical peptide mimicking the receptor binding site. Antibodies raised against this peptide stain the surface of mouse neurons and recognize a 66-kDa membrane protein that binds PrPc both in vitro and in vivo. Furthermore, both the complementary prion peptide and antiserum against it inhibit the toxicity of a prion-derived peptide toward neuronal cells in culture. Such reagents might therefore have therapeutic applications.

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Author notes

  1. Vilma R. Martins and Edgard Graner: These authors contributed equally to this work.

Authors and Affiliations

  1. Fundação Antônio Prudente, Rua Prof. Antônio Prudente, 109/4A, 01509-010, São Paulo, SP, Brazil

    Vilma R. Martins & Silvio M. Zanata

  2. Ludwig Institute for Cancer Research, Rua Prof. Antônio Prudente, 109/4A, 01509-010, São Paulo, SP, Brazil

    Edgard Graner, Sandro J. De Souza, Adriana F. Mercadante, Silvio S. Veiga & Ricardo R. Brentani

  3. Instituto de Ciências Biomédicas, Departamento de Anatomia, Centra de Ciências da Saúde, Bloco F, Ilha do Fundão, UFRJ 21949-900, Rio de Janeiro, Brazil

    José Garcia-Abreu & Vivaldo Moura Neto

  4. Biological Laboratories, Harvard University, Cambridge, Massachusetts, 02138, USA

    Sandro J. De Souza

Authors
  1. Vilma R. Martins
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  2. Edgard Graner
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Martins, V., Graner, E., Garcia-Abreu, J. et al. Complementary hydropathy identifies a cellular prion protein receptor. Nat Med 3, 1376–1382 (1997). https://doi.org/10.1038/nm1297-1376

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