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TNFα regulation of Fas ligand expression on the vascular endothelium modulates leukocyte extravasation

Nature Medicine volume 4, pages 415–420 (1998)Cite this article

Abstract

It is generally believed that the vascular endothelium serves as an inflammatory barrier by providing a nonadherent surface to leukocytes. Here, we report that Fas ligand (FasL) is expressed on vascular endothelial cells (ECs) and that it may function to actively inhibit leukocyte extravasation. TNFα downregulates FasL expression with an accompanying decrease in EC cytotoxicity toward co-cultured Fas-bearing cells. Local administration of TNFα to arteries downregulates endothelial FasL expression and induces mononuclear cell infiltration. Constitutive FasL expression markedly attenuates TNFα-induced cell infiltration and adherent mononuclear cells undergo apoptosis under these conditions. These findings suggest that endothelial FasL expression can negatively regulate leukocyte extravasation.

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Authors and Affiliations

  1. Division of Cardiovascular Research, St. Elizabeth's Medical Center, Tufts University School of Medicine, Boston, Massachusetts, 02135, USA

    Masataka Sata & Kenneth Walsh

  2. Department of Physiology, University of Massachusetts Medical Center, Worcester, MA, 016SS, USA

    Masataka Sata

  3. Program in Cell, Molecular and Developmental Biology, Sackler School of Biomedical Sciences, Tufts University, Boston, Massachusetts, 02111, USA

    Kenneth Walsh

Authors
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Sata, M., Walsh, K. TNFα regulation of Fas ligand expression on the vascular endothelium modulates leukocyte extravasation. Nat Med 4, 415–420 (1998). https://doi.org/10.1038/nm0498-415

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  • DOI: https://doi.org/10.1038/nm0498-415

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