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Reply to: Does a β2-adrenergic receptor–WNK4–Na-Cl co-transporter signal cascade exist in the in vivo kidney?

Nature Medicine volume 18, pages 1325–1327 (2012)Cite this article

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Mu et al. reply:

In their correspondence1, Uchida et al. respond to our paper2 in which we showed that renal β2-adrenergic receptor stimulation downregulates WNK4 expression and upregulates Na-Cl co-transporter (NCC) expression, hence contributing to the development of salt sensitive hypertension. We appreciate the study of Uchida et al.1 and their findings, which confirm our original data that norepinephrine treatment contributes to the development of salt-sensitive hypertension, with a similar efficacy as in our study2. However, they did not detect any changes in WNK4 or NCC mRNA or protein levels in the kidney upon norepinephrine stimulation1.

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Figure 1: Effects of norepinephrine (NE) infusion on renal WNK4 mRNA expression in mice.
Figure 2: Effects of NE treatment on WNK4 and NCC protein expression and evaluation of protein extraction procedures.

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Authors and Affiliations

  1. Research Center for Advanced Science and Technology, The University of Tokyo, Tokyo, Japan

    ShengYu Mu & Toshiro Fujita

  2. The University of Tokyo School of Medicine, Tokyo, Japan

    Tatsuo Shimosawa

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  1. ShengYu Mu
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  2. Tatsuo Shimosawa
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  3. Toshiro Fujita
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Correspondence to Toshiro Fujita.

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Mu, S., Shimosawa, T. & Fujita, T. Reply to: Does a β2-adrenergic receptor–WNK4–Na-Cl co-transporter signal cascade exist in the in vivo kidney?. Nat Med 18, 1325–1327 (2012). https://doi.org/10.1038/nm.2939

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