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Fractional synthesis and clearance rates for amyloid β

Nature Medicine volume 17, pages 1178–1179 (2011)Cite this article

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To the Editor:

We are writing regarding the 2006 publication of a new stable isotope labeling kinetics (SILK) protocol for assessing the fractional synthesis and clearance rates of amyloid β (Aβ) in the brain1. This protocol has been used to demonstrate the relatively accelerated rate of turnover of Aβ peptides in the brain1 and to assess the effect of drugs on Aβ production2. However, we are concerned that aspects of the protocol have not been fully appreciated, raising questions about the interpretation of findings from the protocol.

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Figure 1: During the infusion of 13C6-labeled leucine, the fraction of labeled Aβ secreted by neurons increases from 0% to a theoretical maximum of 10% at the neuronal fractional synthesis rate.

References

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  2. Bateman, R.J. et al. Ann. Neurol. 66, 48–54 (2009).

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  3. Mawuenyega, K.G. et al. Science 330, 1774 (2010).

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Acknowledgements

We would like to acknowledge support from US National Institutes of Health grants AG5131 and AG034439.

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Authors and Affiliations

  1. Department of Family Preventive Medicine, University of California–San Diego, La Jolla, California, USA

    Steven D Edland

  2. Department of Neuroscience, University of California–San Diego, La Jolla, California, USA

    Steven D Edland & Douglas R Galasko

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  1. Steven D Edland
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  2. Douglas R Galasko
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Corresponding author

Correspondence to Steven D Edland.

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Competing interests

The authors declare no competing financial interests.

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Supplementary Results, Supplementary Methods and Supplementary Figures 1 and 2 (PDF 352 kb)

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Edland, S., Galasko, D. Fractional synthesis and clearance rates for amyloid β. Nat Med 17, 1178–1179 (2011). https://doi.org/10.1038/nm.2495

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