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Response to "On the dimerization of CCR5"

Nature Immunology volume 6, pages 535–536 (2005)Cite this article

Hernanz-Falcón et al. reply:

Resonance energy transfer (RET) methods, including BRET and fluorescence RET (FRET), are now widely used to study protein-protein interactions in living cells1. The nature of BRET limits its use to the study of cell populations; it cannot be applied to single-cell analysis. It is thus impossible to determine the cell site at which a signal is generated, and any intracellular associations of the energy transfer partners would be measured as BRET. In contrast, FRET permits visualization of subcellular protein-protein interactions in a single living cell; in this case, background is easily eliminated by the use of an unlabeled cell region as an internal autofluorescence reference.

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References

  1. Pfleger, K.D.G. & Eidne, K.A. Biochem. J. 385, 625–637 (2005).

    Article  CAS  Google Scholar 

  2. Kenworthy, A.K., Petranova, N. & Edidin, M. Mol. Biol. Cell 11, 1645–1655 (2000).

    Article  CAS  Google Scholar 

  3. Ramsay, D., Kellet, E., McVey, M., Rees, S. & Milligan, G. Biochem. J. 365, 429–440 (2002).

    Article  CAS  Google Scholar 

  4. Percherancier, Y. et al. J. Biol. Chem. 280, 9895–9903 (2005).

    Article  CAS  Google Scholar 

  5. Toth, P.T., Ren, D. & Miller, R.J. J. Pharmacol. Exp. Ther. 310, 8–17 (2004).

    Article  CAS  Google Scholar 

Download references

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Authors and Affiliations

  1. Department of Immunology and Oncology, Centro Nacional de Biotecnología–Consejo Superior de Investigaciones Científicas, Darwin, 3, Campus Universitario de Cantoblanco, Madrid, E-28049, Spain

    Patricia Hernanz-Falcón, José Miguel Rodríguez-Frade, Antonio Serrano, Carlos Martínez-A & Mario Mellado

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  1. Patricia Hernanz-Falcón
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  2. José Miguel Rodríguez-Frade
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  3. Antonio Serrano
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  4. Carlos Martínez-A
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  5. Mario Mellado
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Correspondence to Carlos Martínez-A.

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Hernanz-Falcón, P., Rodríguez-Frade, J., Serrano, A. et al. Response to "On the dimerization of CCR5". Nat Immunol 6, 535–536 (2005). https://doi.org/10.1038/ni0605-535b

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